Human HLA-B27 ELISA Kit (Colorimetric)

Novus Biologicals | Catalog # NBP3-00395

Novus Biologicals
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Key Product Details

Sample Type & Volume Required Per Well

Serum, plasma and other biological fluids (100 ul)

Sensitivity

0.19 ng/mL

Assay Range

0.31 - 20 ng/mL
View all HLA-B27 ELISA Kits »

Product Specifications

Assay Type

Sandwich-ELISA

Kit Type

ELISA Kit (Colorimetric)

Species

Human

精度

Intra-Assay Precision (Precision within an assay) CV 6.06%

Inter-Assay Precision (Precision between assays) CV 5.24%

Recovery for Human HLA-B27 ELISA Kit (Colorimetric)

Recovery

Serum (n=8): 97%
EDTA plasma (n=8): 93%
Cell culture media(n=8): 96%

Linearity

Human HLA B ELISA Kit (Colorimetric)

Scientific Data Images for Human HLA-B27 ELISA Kit (Colorimetric)

Human HLA B ELISA Kit (Colorimetric)

ELISA: Human HLA B ELISA Kit (Colorimetric) [NBP3-00395] -

ELISA: Human HLA B ELISA Kit (Colorimetric) [NBP3-00395] - Standard Curve Reference

Kit Contents for Human HLA-B27 ELISA Kit (Colorimetric)

  • Biotinylated Detection Ab Diluent
  • Concentrated Biotinylated Detection Ab (100x)
  • Concentrated HRP Conjugate (100x)
  • Concentrated Wash Buffer (25x)
  • HRP Conjugate Diluent
  • Micro ELISA Plate (Dismountable)
  • Plate Sealer
  • Product Manual
  • Reference Standard
  • Sample Diluent
  • Stop Solution
  • Substrate Reagent

Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Storage of components varies. See protocol for specific instructions.

Background: HLA-B27

HLA-B belongs to the HLA class I heavy chain paralogues. This class I molecule is a heterodimer consisting of a heavy chain and a light chain (beta-2 microglobulin). The heavy chain is anchored in the membrane. Class I molecules play a central role in the immune system by presenting peptides derived from the endoplasmic reticulum lumen. They are expressed in nearly all cells. The heavy chain is approximately 45 kDa and its gene contains 8 exons. Exon 1 encodes the leader peptide, exon 2 and 3 encode the alpha1 and alpha2 domains, which both bind the peptide, exon 4 encodes the alpha3 domain, exon 5 encodes the transmembrane region and exons 6 and 7 encode the cytoplasmic tail. Polymorphisms within exon 2 and exon 3 are responsible for the peptide binding specificity of each class one molecule. Typing for these polymorphisms is routinely done for bone marrow and kidney transplantation. Hundreds of HLA-B alleles have been described. [provided by RefSeq, Jul 2008]

Alternate Names

HLA class I histocompatibility antigen, B alpha chain, human leukocyte antigen B27, leukocyte antigen class I-B, major histocompatibility complex, class I, B, MHC class I antigen HLA-B heavy chain, MHC HLA-B cell surface glycoprotein

Gene Symbol

HLA-B

Additional HLA-B27 Products

Product Documents for Human HLA-B27 ELISA Kit (Colorimetric)

Certificate of Analysis

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Product Specific Notices for Human HLA-B27 ELISA Kit (Colorimetric)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

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