HOXC4 Antibody (1E9) - Azide and BSA Free
Novus Biologicals | Catalog # H00003221-M01
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Key Product Details
Species Reactivity
Human
Applications
Western Blot, ELISA, Knockdown Validated
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG2a Kappa Clone # 1E9
Format
Azide and BSA Free
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Product Specifications
Immunogen
HOXC4 (NP_705897, 160 a.a. ~ 264 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. RTAYTRQQVLELEKEFHYNRYLTRRRRIEIAHSLCLSERQIKIWFQNRRMKWKKDHRLPNTKVRSAPPAGAAPSTLSAATPGTSEDHSQSATPPEQQRAEDITRL
Specificity
HOXC4 - homeo box C4
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2a Kappa
Description
Quality control test: Antibody Reactive Against Recombinant Protein.
Scientific Data Images for HOXC4 Antibody (1E9) - Azide and BSA Free
Western Blot: HOXC4 Antibody (1E9) [H00003221-M01]
Western Blot: HOXC4 Antibody (1E9) [H00003221-M01] - Analysis of HOXC4 expression in transfected 293T cell line by HOXC4 monoclonal antibody (M01), clone 1E9.Lane 1: HOXC4 transfected lysate(29.8 KDa).Lane 2: Non-transfected lysate.Western Blot: HOXC4 Antibody (1E9) [H00003221-M01]
Western Blot: HOXC4 Antibody (1E9) [H00003221-M01] - Analysis of HOXC4 over-expressed 293 cell line, cotransfected with HOXC4 Validated Chimera RNAi ( Cat # H00003221-R01V ) (Lane 2) or non-transfected control (Lane 1). Blot probed with HOXC4 monoclonal antibody (M01), clone 1E9 (Cat # H00003221-M01 ). GAPDH ( 36.1 kDa ) used as specificity and loading control.Western Blot: HOXC4 Antibody (1E9) [H00003221-M01]
Western Blot: HOXC4 Antibody (1E9) [H00003221-M01] - HOXC4 monoclonal antibody (M01), clone 1E9 Analysis of HOXC4 expression in A-549.ELISA: HOXC4 Antibody (1E9) [H00003221-M01]
ELISA: HOXC4 Antibody (1E9) [H00003221-M01] - Detection limit for recombinant GST tagged HOXC4 is approximately 0.1ng/ml as a capture antibody.Applications for HOXC4 Antibody (1E9) - Azide and BSA Free
Application
Recommended Usage
Western Blot
1:500
Application Notes
Antibody reactivity against cell lysate and recombinant protein for WB. It has been used for RNAi Validation and ELISA.
Formulation, Preparation, and Storage
Purification
IgG purified
Formulation
In 1x PBS, pH 7.4
Format
Azide and BSA Free
Preservative
No Preservative
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Background: HOXC4
Alternate Names
homeo box 3E, homeo box C4, homeobox C4, Homeobox protein CP19, Homeobox protein Hox-3E, homeobox protein Hox-C4, HOX3, HOX3Ecp19
Entrez Gene IDs
3221 (Human)
Gene Symbol
HOXC4
OMIM
142974 (Human)
UniProt
Additional HOXC4 Products
Product Documents for HOXC4 Antibody (1E9) - Azide and BSA Free
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Product Specific Notices for HOXC4 Antibody (1E9) - Azide and BSA Free
This product is produced by and distributed for Abnova, a company based in Taiwan.
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- Cellular Response to Hypoxia Protocols
- ELISA Sample Preparation & Collection Guide
- ELISA Troubleshooting Guide
- How to Run an R&D Systems DuoSet ELISA
- How to Run an R&D Systems Quantikine ELISA
- How to Run an R&D Systems Quantikine™ QuicKit™ ELISA
- Quantikine HS ELISA Kit Assay Principle, Alkaline Phosphatase
- Quantikine HS ELISA Kit Principle, Streptavidin-HRP Polymer
- R&D Systems Quality Control Western Blot Protocol
- Sandwich ELISA (Colorimetric) – Biotin/Streptavidin Detection Protocol
- Sandwich ELISA (Colorimetric) – Direct Detection Protocol
- Troubleshooting Guide: ELISA
- Troubleshooting Guide: Western Blot Figures
- Western Blot Conditions
- Western Blot Protocol
- Western Blot Protocol for Cell Lysates
- Western Blot Troubleshooting
- Western Blot Troubleshooting Guide
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
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