Human 17 beta-HSD1/HSD17B1 Antibody (860020)

R&D Systems | Catalog # MAB7178

R&D Systems

Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Validated:

Western Blot, Simple Western

Cited:

Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2B Clone # 860020
View all 17 beta-HSD1/HSD17B1 Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant human 17 beta ‑HSD1/HSD17B1
Ala2-Gln328
Accession # P14061

Specificity

Detects human 17 beta ‑HSD1/HSD17B1 in ELISAs.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2B

Scientific Data Images for Human 17 beta-HSD1/HSD17B1 Antibody (860020)

Detection of Human 17 beta -HSD1/ HSD17B1 antibody by Western Blot.

Detection of Human 17 beta ‑HSD1/ HSD17B1 by Western Blot.

Western blot shows lysates of human placenta tissue. PVDF membrane was probed with 0.25 µg/mL of Mouse Anti-Human 17 beta -HSD1/HSD17B1 Monoclonal Antibody (Catalog # MAB7178) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for 17 beta -HSD1/HSD17B1 at approximately 35 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Detection of Human 17 beta -HSD1/HSD17B1 antibody by Simple WesternTM.

Detection of Human 17 beta ‑HSD1/HSD17B1 by Simple WesternTM.

Simple Western lane view shows lysates of human placenta tissue, loaded at 0.2 mg/mL. A specific band was detected for 17 beta ‑HSD1/HSD17B1 at approximately 40 kDa (as indicated) using 2.5 µg/mL of Mouse Anti-Human 17 beta ‑HSD1/HSD17B1 Monoclonal Antibody (Catalog # MAB7178). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system. Non-specific interaction with the 230 kDa Simple Western standard may be seen with this antibody.

Applications for Human 17 beta-HSD1/HSD17B1 Antibody (860020)

Application
Recommended Usage

Simple Western

2.5 µg/mL
Sample: Human placenta tissue

Western Blot

0.25 µg/mL
Sample: Human placenta tissue

Reviewed Applications

Read 1 review rated 4 using MAB7178 in the following applications:

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Sterile PBS to a final concentration of 0.5 mg/mL. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: 17 beta-HSD1/HSD17B1

HSD17B1 (Hydroxysteroid 17-beta dehydrogenase type 1; also 17  beta ‑HSD1 and E2DH) is a 33-34 kDa member of the short chain dehydrogenase/reductase (SDR) superfamily of molecules. It is a cytoplasmic breast, placental and gonadal enzyme that both converts DHEA to estrogen A-diol, and inactivates DHT through oxidation and reduction. Human HSD17B1 is 328 amino acids (aa) in length. It contains an NADP binding site (aa 10-38) and a steroid catalytic site (aa 210-221). HSD17B1 functions as a 68 kDa noncovalently-linked homodimer. Over aa 2-328, human HSD17B1 shares 65% aa identity with mouse HSD17B1.

Long Name

17 beta Hydroxysteroid Dehydrogenase 1

Alternate Names

17 betaHSD1, E2DH, EDH17B2, EDHB17, HSD17B1, SDR28C1

Entrez Gene IDs

3292 (Human); 15485 (Mouse); 25322 (Rat)

Gene Symbol

HSD17B1

UniProt

P14061 (Human)

Additional 17 beta-HSD1/HSD17B1 Products

Product Documents for Human 17 beta-HSD1/HSD17B1 Antibody (860020)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human 17 beta-HSD1/HSD17B1 Antibody (860020)

For research use only

Citations for Human 17 beta-HSD1/HSD17B1 Antibody (860020)

Customer Reviews for Human 17 beta-HSD1/HSD17B1 Antibody (860020) (1)

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  • Human 17 beta -HSD1/HSD17B1 Antibody
    Name: Anonymous
    Application: Western Blot
    Sample Tested: Endometrial epithelial
    Species: Human
    Verified Customer | Posted 03/27/2017
    Human 17 beta-HSD1/HSD17B1 Antibody (860020) MAB7178

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