Human ADAM19 Alexa Fluor® 647-conjugated Antibody

Catalog #: AF5050R Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
AF5050R-100UG

Save 15% on Select RUO Reagents. See Details.

All ADAM19 Antibodies

Product Details

Supplemental Products

Reviews

Summary Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human ADAM19 Alexa Fluor® 647-conjugated Antibody Summary

Species Reactivity

Human

Specificity

Detects human ADAM19 in direct ELISAs and Western blots. In direct ELISAs and Western blots, approximately 25% cross-reactivity with recombinant mouse ADAM19 is observed, and less than 5% cross-reactivity with recombinant human (rh)&nb

Source

Polyclonal Sheep IgG

Purification

Antigen Affinity-purified

Immunogen

Chinese hamster ovary cell line CHO-derived recombinant human ADAM19
Arg26-Gln501 (Ser32 del) (Lys33Arg)
Accession # Q9H013

Formulation

Supplied 0.2mg/ml in 1X PBS with RDF1 and 0.09% Sodium Azide

Label

Alexa Fluor 647 (Excitation= 650 nm, Emission= 668 nm)

Applications

Recommended Concentration

Sample

Western Blot

Optimal dilution of this antibody should be experimentally determined.

Immunoprecipitation

Optimal dilution of this antibody should be experimentally determined.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied

Background: ADAM19

ADAM19 (a disintegrin and metalloprotease 19; also MADDAM and Meltrin-beta ) is a 95‑100 kDa member of the M12B peptidase family of enzymes. It is expressed on multiple cell types including monocytes, fibroblasts, osteoblasts and dendritic cells. The human ADAM19 proenzyme is a 931 amino acid (aa) type I transmembrane protein. It contains a 178 aa cleavable proregion (aa 26‑203) and a 497 aa extracellular domain (ECD) (aa 204‑702). The ECD contains a metalloprotease domain (aa 211‑409), a disintegrin region (aa 417‑503) and a Cys-rich domain (aa 504‑650). The ECD circulates in plasma bound to 700 kDa alpha 2-Macroglobulin. One splice variant shows an alternate start site at Met71, while two isoforms show variations after Lys902. Over aa 205‑702, human ADAM19 is 87% aa identical to mouse ADAM19.

Long Name

A Disintegrin and Metalloprotease-like Domain 19

Entrez Gene IDs

8728 (Human); 11492 (Mouse)

Alternate Names

a disintegrin and metalloproteinase domain 19 (meltrin beta); ADAM 19; ADAM metallopeptidase domain 19; ADAM19; disintegrin and metalloproteinase domain-containing protein 19; EC 3.4.24; EC 3.4.24.-; MADDAM; Meltrin beta; meltrin-beta; Metalloprotease and disintegrin dendritic antigen marker; MLTNBmeltrin beta