Type-1 angiotensin II receptor, AGTR1, is a seven transmembrane domain (7TM) G protein-coupled receptor (GPCR) that mediates the cardiovascular effects of angiotensin II. At least four transcript variants have been described for the AGTR1 gene.
Human AGTR-1 Antibody (1010106)
R&D Systems | Catalog # MAB102441
Key Product Details
Species Reactivity
Human
Applications
Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG2B Clone # 1010106
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Product Specifications
Immunogen
Synthetic peptide containing human AGTR-1
Accession # P30556
Accession # P30556
Specificity
Detects human AGTR-1 in direct ELISAs and Western blots.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2B
Scientific Data Images for Human AGTR-1 Antibody (1010106)
Detection of Human AGTR‑1 by Western Blot.
Western blot shows lysates of human liver tissue and human lung tissue. PVDF membrane was probed with 1 µg/mL of Mouse Anti-Human AGTR-1 Monoclonal Antibody (Catalog # MAB102441) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (HAF018). A specific band was detected for AGTR-1 at approximately 43 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.Applications for Human AGTR-1 Antibody (1010106)
Application
Recommended Usage
Western Blot
1 µg/mL
Sample: Human liver tissue and Human lung tissue
Sample: Human liver tissue and Human lung tissue
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
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Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Calculators
Background: AGTR-1
Long Name
Angiotensin II Receptor Type 1
Alternate Names
AG2S, AGTR1, AT1, AT1AR, AT1BR, AT1R, AT2R1
Gene Symbol
AGTR1
UniProt
Additional AGTR-1 Products
Product Documents for Human AGTR-1 Antibody (1010106)
Certificate of Analysis
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Note: Certificate of Analysis not available for kit components.
Product Specific Notices for Human AGTR-1 Antibody (1010106)
For research use only
Related Research Areas
Citations for Human AGTR-1 Antibody (1010106)
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- Cellular Response to Hypoxia Protocols
- R&D Systems Quality Control Western Blot Protocol
- Troubleshooting Guide: Western Blot Figures
- Western Blot Conditions
- Western Blot Protocol
- Western Blot Protocol for Cell Lysates
- Western Blot Troubleshooting
- Western Blot Troubleshooting Guide
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
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Associated Pathways
