Human Aminoacylase/ACY1 Alexa Fluor® 488-conjugated Antibody

Catalog #: FAB2900G Datasheet / COA / SDS

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FAB2900G-100UG

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Human Aminoacylase/ACY1 Alexa Fluor® 488-conjugated Antibody Summary

Species Reactivity

Human

Specificity

Detects recombinant human Aminoacylase/ACY1 in direct ELISAs and Western blots. In Western blots, approximately 50% cross-reactivity with recombinant mouse ACY1 is observed.

Source

Monoclonal Rat IgG2b Clone # 475626

Purification

Protein A or G purified

Immunogen

S. frugiperda insect ovarian cell line Sf 21-derived recombinant human Aminoacylase/ACY1
Thr2-Ser408
Accession # Q03154

Formulation

Supplied 0.2mg/ml in 1X PBS with RDF1 and 0.09% Sodium Azide

Label

Alexa Fluor 488 (Excitation= 488 nm, Emission= 515-545 nm)

Applications

Recommended Concentration

Sample

Western Blot

Optimal dilution of this antibody should be experimentally determined.

Immunoprecipitation

Optimal dilution of this antibody should be experimentally determined.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

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Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied

Background: Aminoacylase/ACY1

The human ACY1 gene encodes Aminoacylase, a member of the M20 family of metalloproteases (1). ACY1 plays a general role in the cytosolic breakdown of acetylated amino acids generated during protein degradation. It also interacts with sphingosine kinase type 1, which is involved in promoting cell growth and inhibiting apoptosis of tumor cells (2). The full-length protein is expressed and the purified enzyme is active as described in Activity Assay Protocol.

Entrez Gene IDs

95 (Human); 109652 (Mouse)

Alternate Names

ACY1; ACY-1; ACY1D; acylase; aminoacylase 1; Aminoacylase; aminoacylase-1; EC 3.5.1; EC 3.5.1.14; N-acyl-L-amino-acid amidohydrolase