Human AP-2 beta Alexa Fluor® 594-conjugated Antibody

Catalog #: AF5634T Datasheet / COA / SDS

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AF5634T-100UG

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All AP-2 beta Antibodies

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Human AP-2 beta Alexa Fluor® 594-conjugated Antibody Summary

Species Reactivity

Human

Specificity

Detects endogenous human AP-2 beta in Western blots.

Source

Polyclonal Goat IgG

Purification

Antigen Affinity-purified

Immunogen

E. coli-derived recombinant human AP-2Î²
Leu121-Phe218
Accession # Q92481

Formulation

Supplied 0.2mg/ml in 1X PBS with RDF1 and 0.09% Sodium Azide

Label

Alexa Fluor 594 (Excitation= 590 nm, Emission= 617 nm)

Applications

Recommended Concentration

Sample

Western Blot

Optimal dilution of this antibody should be experimentally determined.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied

Background: AP-2 beta

AP-2 beta (Activating enhancer-binding protein 2-beta; also TFAP2B) is a 52 kDa member of the AP-2 family of transcription factors. It is primarily a fetal-expressed protein, being found in renal and skin epithelial cells, as well as neuroblasts plus cells of the facial mesenchyme. In adults, AP-2 beta is expressed in renal epithelium. Human AP-2 beta is 460 amino acids (aa) in length. It contains a transactivation domain (aa 41‑131), a DNA-binding motif (aa 132‑298) and a C-terminal dimerization region (aa 299‑439). SUMOylation occurs on Lys21, adding 15 kDa of MW in SDS-PAGE. AP-2 beta both homodimerizes, and heterodimerizes with AP-2 alpha and -2 gamma. Dimers appear important for facial and limb development.

Long Name

Activating Enhancer-binding Protein 2 beta

Entrez Gene IDs

7021 (Human); 21419 (Mouse); 301285 (Rat)

Alternate Names

activating enhancer binding protein 2 beta; Activating enhancer-binding protein 2-beta; AP2 beta; AP-2 beta; AP-2B; AP2-B; AP2-beta; CHAR; MGC21381; TFAP2B; transcription factor AP-2 beta (activating enhancer binding protein 2 beta); transcription factor AP-2 beta (activating enhancer-binding protein 2 beta); transcription factor AP-2-beta