Human AP-2 beta Antibody

R&D Systems | Catalog # AF5634

R&D Systems

Key Product Details

Species Reactivity

Human

Applications

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG
View all AP-2 beta Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant human AP-2 beta
Leu121-Phe218
Accession # Q92481

Specificity

Detects endogenous human AP-2 beta in Western blots.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for Human AP-2 beta Antibody

Detection of Human AP‑2 beta antibody by Western Blot.

Detection of Human AP‑2 beta by Western Blot.

Western blot shows lysates of SH-SY5Y human neuroblastoma cell line. PVDF membrane was probed with 1 µg/mL of Human AP-2 beta Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5634) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). A specific band was detected for AP-2 beta at approximately 55 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Applications for Human AP-2 beta Antibody

Application
Recommended Usage

Western Blot

1 µg/mL
Sample: SH-SY5Y human neuroblastoma cell line

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: AP-2 beta

AP-2 beta (Activating enhancer-binding protein 2-beta; also TFAP2B) is a 52 kDa member of the AP-2 family of transcription factors. It is primarily a fetal-expressed protein, being found in renal and skin epithelial cells, as well as neuroblasts plus cells of the facial mesenchyme. In adults, AP-2 beta is expressed in renal epithelium. Human AP-2 beta is 460 amino acids (aa) in length. It contains a transactivation domain (aa 41‑131), a DNA-binding motif (aa 132‑298) and a C-terminal dimerization region (aa 299‑439). SUMOylation occurs on Lys21, adding 15 kDa of MW in SDS-PAGE. AP-2 beta both homodimerizes, and heterodimerizes with AP-2 alpha and -2 gamma. Dimers appear important for facial and limb development.

Long Name

Activating Enhancer-binding Protein 2 beta

Alternate Names

AP2 beta, CHAR, TFAP2B

Entrez Gene IDs

7021 (Human); 21419 (Mouse); 301285 (Rat)

Gene Symbol

TFAP2B

UniProt

Q92481

Additional AP-2 beta Products

Product Documents for Human AP-2 beta Antibody

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human AP-2 beta Antibody

For research use only

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