Human Apolipoprotein C-II/ApoC2 Alexa Fluor® 700-conjugated Antibody

Catalog #: AF4497N Datasheet / COA / SDS

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AF4497N-100UG

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Human Apolipoprotein C-II/ApoC2 Alexa Fluor® 700-conjugated Antibody Summary

Species Reactivity

Human

Specificity

Detects human ApoC2 in Western blots. In Western blots, this antibody detects both the precursor and processed forms of ApoC2.

Source

Polyclonal Sheep IgG

Purification

Antigen Affinity-purified

Immunogen

E. coli-derived recombinant human ApoC2
Thr23-Glu101
Accession # P02655

Formulation

Supplied 0.2mg/ml in 1X PBS with RDF1 and 0.09% Sodium Azide

Label

Alexa Fluor 700 (Excitation= 675-700 nm, Emission= 723 nm)

Applications

Recommended Concentration

Sample

Western Blot

Optimal dilution of this antibody should be experimentally determined.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

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Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied

Background: Apolipoprotein C-II/ApoC2

Apolipoprotein C2 (ApoC2/II) is a 8‑9 kDa, secreted member of the Apolipoprotein C2 family of proteins. It is produced by hepatocytes and represents a major component of VLDL particles. It activates lipoprotein lipase and may self-associate to form amyloid-type fibrils. The human ApoC2 precursor is 101 amino acids (aa) in length. It contains a 22 amino acid (aa) signal sequence, followed by a 79 aa ProApoC2 that contains a lipid-binding region (aa 43‑51) and an enzyme interaction site (aa 55‑78). ProApoC2 represents >90% of circulating ApoC2. In human, limited proteolytic processing occurs with removal of the six aa prosegment (aa 23‑28). This does not affect activity. Human ProApoC2 is 59% and 62% aa identical to mouse and rat ProApoC2, respectively.

Entrez Gene IDs

344 (Human); 11813 (Mouse); 292697 (Rat)

Alternate Names

APC2; APOC2; Apo-CII; apoC-II; Apolipoprotein C2; Apolipoprotein CII; Apolipoprotein C-II; MGC75082