Human Apolipoprotein C-II/ApoC2 Antibody

R&D Systems | Catalog # AF4497

R&D Systems

Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Validated:

Western Blot, Simple Western

Cited:

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Sheep IgG
View all Apolipoprotein C-II/ApoC2 Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant human ApoC2
Thr23-Glu101
Accession # P02655

Specificity

Detects human ApoC2 in Western blots. In Western blots, this antibody detects both the precursor and processed forms of ApoC2.

Clonality

Polyclonal

Host

Sheep

Isotype

IgG

Scientific Data Images for Human Apolipoprotein C-II/ApoC2 Antibody

Detection of Human Apolipoprotein C-II/ApoC2 antibody by Western Blot.

Detection of Human Apolipoprotein C-II/ApoC2 by Western Blot.

Western blot shows lysates of human small intestine tissue and plasma. PVDF membrane was probed with 0.5 µg/mL of Human Apolipoprotein C-II/ApoC2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4497) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for precursor Apolipoprotein C-II/ApoC2 at approximately 12 kDa and processed ApoC2 at approximately 8 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 2.
Detection of Human Apolipoprotein C-II/ApoC2 antibody by Simple WesternTM.

Detection of Human Apolipoprotein C‑II/ApoC2 by Simple WesternTM.

Simple Western lane view shows lysates of human plasma, loaded at 0.2 mg/mL. A specific band was detected for Apolipoprotein C-II/ApoC2 at approximately 9 kDa (as indicated) using 5 µg/mL of Sheep Anti-Human Apolipoprotein C-II/ApoC2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4497) followed by 1:50 dilution of HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.

Applications for Human Apolipoprotein C-II/ApoC2 Antibody

Application
Recommended Usage

Simple Western

5 µg/mL
Sample: Human plasma

Western Blot

0.5 µg/mL
Sample: Human small intestine tissue and plasma

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Apolipoprotein C-II/ApoC2

Apolipoprotein C2 (ApoC2/II) is a 8‑9 kDa, secreted member of the Apolipoprotein C2 family of proteins. It is produced by hepatocytes and represents a major component of VLDL particles. It activates lipoprotein lipase and may self-associate to form amyloid-type fibrils. The human ApoC2 precursor is 101 amino acids (aa) in length. It contains a 22 amino acid (aa) signal sequence, followed by a 79 aa ProApoC2 that contains a lipid-binding region (aa 43‑51) and an enzyme interaction site (aa 55‑78). ProApoC2 represents >90% of circulating ApoC2. In human, limited proteolytic processing occurs with removal of the six aa prosegment (aa 23‑28). This does not affect activity. Human ProApoC2 is 59% and 62% aa identical to mouse and rat ProApoC2, respectively.

Alternate Names

APOC2, Apolipoprotein CII

Entrez Gene IDs

344 (Human); 11813 (Mouse); 292697 (Rat)

Gene Symbol

APOC2

UniProt

P02655

Additional Apolipoprotein C-II/ApoC2 Products

Product Documents for Human Apolipoprotein C-II/ApoC2 Antibody

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human Apolipoprotein C-II/ApoC2 Antibody

For research use only

Related Research Areas

Obesity

Citations for Human Apolipoprotein C-II/ApoC2 Antibody

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