Key Product Details

Species Reactivity

Human

Applications

Western Blot, Neutralization

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2B Clone # 136716
View all B7-H2 Primary Antibodies »

Product Specifications

Immunogen

Mouse myeloma cell line NS0-derived recombinant human B7-H2
Asp19-Ser258
Accession # O75144

Specificity

Detects human B7-H2 in direct ELISAs and Western blots. In direct ELISAs and Western blots, no cross-reactivity with recombinant human (rh) B7-1, rhB7-2, rhB7-H1, rhB7-H3, recombinant mouse (rm) B7-H2, or rmPD-L2 is observed.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2B

Endotoxin Level

<0.10 EU per 1 μg of the antibody by the LAL method.

Scientific Data Images for Human B7-H2 Antibody (136716)

Cell Proliferation Induced by B7‑H2 and Neutralization by Human B7‑H2 Antibody.

Cell Proliferation Induced by B7‑H2 and Neutralization by Human B7‑H2 Antibody.

In the presence of Human CD3e Monoclonal Antibody (20 ng/mL, Catalog # MAB100), Recombinant Human B7-H2 Fc Chimera (Catalog # 165-B7) stimulates proliferation in PHA-activated human T cells in a dose-dependent manner (orange line). Under these conditions, proliferation elicited by Rec-ombinant Human B7-H2 Fc Chimera (3 µg/mL) is neutralized (green line) by increasing concentrations of Mouse Anti-Human B7-H2 Monoclonal Antibody (Catalog # MAB1651). The ND50 is typically 1-4 µg/mL.

Applications for Human B7-H2 Antibody (136716)

Application
Recommended Usage

Western Blot

1 µg/mL
Sample: Recombinant Human B7-H2 Fc Chimera (Catalog # 165-B7)

Neutralization

Measured by its ability to neutralize B7‑H2-induced proliferation in PHA-activated human T cells. Wang, S. et al. (2000) Blood 96:2808. The Neutralization Dose (ND50) is typically 1-4 µg/mL in the presence of 3 µg/mL Recombinant Human B7‑H2 Fc Chimera and 20 ng/mL Human CD3 epsilon Monoclonal Antibody.

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: B7-H2

Human B7-H2, also called B7RP-1, B7h, LICOS, and GL50, is a 60 kDa member of the B7 family of immune costimulatory proteins, which includes B7-1, B7-2, B7-H1 (PD-L1), PD-L2, and B7-H3. B7 proteins are members of the immunoglobulin (Ig) superfamily; the extracellular domains contain 2 Ig-like domains and all members have short cytoplasmic domains. Family members share about 20‑25% amino acid identity. Within the extracellular domain, human B7-H2 shares 49% and 54% amino acid sequence identity with mouse and rat B7-H2, respectively. B7-H2 has been identified as the ligand for ICOS, a member of the CD28 family of costimulatory receptors. Human B7-H2 is a 309 amino acid (aa) protein with a putative 18 aa signal peptide, a 239 aa extracellular domain, an 18 aa transmembrane region, and a 33 aa cytoplasmic domain. Human B7-H2 is expressed constitutively on resting B cells, dendritic cells, and at low levels on monocytes. The B7-H2/ICOS interaction appears to play roles in T cell dependent B cell activation and Th differentiation.

References

  1. Coyle, A.J. and J.C. Gutierrez-Ramos (2001) Nat. Immunol. 2:203.
  2. Ling, V. et al. (2000) J. Immunol. 164:1653.
  3. Wang, S. et al. (2000) Blood 96:2808.
  4. Brodie, D. et al. (2000) Curr. Biol. 10:333.
  5. Mages, H.W. et al. (2000) Eur. J. Immunol. 30:1040.
  6. Swallow, M.M. et al. (1999) Immunity 11:423.
  7. Yoshinaga, S.K. et al. (1999) Nature 402:827.

Long Name

B7 Homolog 2

Alternate Names

B7H2, B7RP-1, CD275, GL50, ICOSL, ICOSLG

Entrez Gene IDs

23308 (Human); 50723 (Mouse); 499415 (Rat); 102132485 (Cynomolgus Monkey)

Gene Symbol

ICOSLG

UniProt

O75144

Additional B7-H2 Products

Product Documents for Human B7-H2 Antibody (136716)

Certificate of Analysis

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Product Specific Notices for Human B7-H2 Antibody (136716)

For research use only

Citations for Human B7-H2 Antibody (136716)

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