Human beta 2-Microglobulin Parameter Assay Kit

R&D Systems | Catalog # KGE019

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Key Product Details

Assay Length

1.5 hours

Sample Type & Volume Required Per Well

Serum (20 µL), EDTA Plasma (20 µL), Heparin Plasma (20 µL), Urine (10 µL)

Sensitivity

0.132 µg/mL

Assay Range

0.15-9.6 µg/mL (Serum, EDTA Plasma, Heparin Plasma, Urine)
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Product Summary for Human beta 2-Microglobulin Parameter Assay Kit

The Parameter beta 2-Microglobulin Immunoassay is a 1.5 hour forward sequential competitive enzyme immunoassay designed to measure beta 2M in serum, plasma, and urine.

Product Specifications

Measurement

Quantitative ELISA

Detection Method

Colorimetric - 450nm (TMB)

Conjugate

Biotin

Species

Human

Specificity

Measures Human beta 2M levels in various samples.

Cross-reactivity

< 0.5% cross-reactivity observed with available related molecules. < 50% cross-species reactivity observed with species tested.

Interference

No significant interference observed with available related molecules.

精度

Intra-Assay Precision (Precision within an assay) Three controls of known concentration were tested twenty times on one plate to assess intra-assay precision.

Inter-Assay Precision (Precision between assays) Three controls of known concentration were tested in twenty separate assays to assess inter-assay precision. Assays were performed by at least three technicians using two lots of components.

EDTA Plasma, Heparin Plasma, Serum, Urine

Intra-Assay Precision Inter-Assay Precision
Sample 1 2 3 1 2 3
n 20 20 20 20 20 20
Mean (µg/mL) 1.14 3.19 6.41 0.953 3.10 6.17
Standard Deviation 0.086 0.189 0.383 0.175 0.293 0.496
CV% 7.5 5.9 6.0 18.4 9.5 8.0

Recovery for Human beta 2-Microglobulin Parameter Assay Kit

The recovery of human beta 2M spiked to levels throughout the range of the assay in various matrices was evaluated.

Sample Type Average % Recovery Range %
EDTA Plasma (n=4) 99 79-114
Heparin Plasma (n=4) 106 91-124
Serum (n=4) 101 85-122
Urine (n=4) 90 76-105

Linearity

To assess the linearity of the assay, samples containing and/or spiked with high concentrations of human beta 2M were serially diluted with calibrator diluent to produce samples with values within the dynamic range of the assay.

Human beta 2-Microglobulin Assay Linearity

Scientific Data Images for Human beta 2-Microglobulin Parameter Assay Kit

Human beta 2-Microglobulin Assay ELISA Standard Curve

Human beta 2-Microglobulin Assay ELISA Standard Curve

Preparation and Storage

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: beta 2-Microglobulin

beta 2-Microglobulin (B2M) forms the light chain of Class I MHC molecules. It has been measured in a variety of body fluids, including serum/plasma, saliva, CSF, and urine. B2M freely passes through the glomerular membrane, but it is nearly completely reabsorbed and degraded in proximal tubule cells. Clinically, serum B2M is elevated in rheumatoid arthritis, systemic lupus erythematosus, viral infections, and conditions associated with decreased glomerular filtration.

Alternate Names

B2M, beta 2Microglobulin

Entrez Gene IDs

567 (Human); 12010 (Mouse); 24223 (Rat); 101867173 (Cynomolgus Monkey)

Gene Symbol

B2M

Additional beta 2-Microglobulin Products

Product Documents for Human beta 2-Microglobulin Parameter Assay Kit

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

Product Specific Notices for Human beta 2-Microglobulin Parameter Assay Kit

For research use only

⚠ WARNING: This product can expose you to chemicals including N,N-Dimethylforamide, which is known to the State of California to cause cancer. For more information, go to www.P65Warnings.ca.gov.

Related Research Areas

Cancer Biomarkers

Citations for Human beta 2-Microglobulin Parameter Assay Kit

Customer Reviews for Human beta 2-Microglobulin Parameter Assay Kit (2)

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  • Human beta 2-Microglobulin Parameter Assay Kit
    Name: Anonymous
    Sample Tested: Serum and Plasma
    Verified Customer | Posted 07/06/2021
    we used this kit to quantify beta 2 microglobulin in human serum and plasma samples. Works very well and easy to use protocol.
    Human beta 2-Microglobulin Parameter Assay Kit KGE019
  • Human beta 2-Microglobulin Parameter Assay Kit
    Name: Anonymous
    Sample Tested: Human Dialysate
    Verified Customer | Posted 08/23/2016

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