Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Validated:

Western Blot, Simple Western

Cited:

Flow Cytometry

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG
View all c-Abl Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant human c-Abl
Ala941-Val1140
Accession # NP_009297

Specificity

Detects human c-Abl in Western blots.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for Human c‑Abl Antibody

Detection of Human c-Abl antibody by Western Blot.

Detection of Human c-Abl by Western Blot.

Western blot shows lysates of Jurkat human acute T cell leukemia cell line, MCF-7 human breast cancer cell line, and MDA-MB-453 human breast cancer cell line. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human c-Abl Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5414) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). A specific band was detected for c-Abl at approximately 135 - 145 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Detection of Human c‑Abl antibody by Simple WesternTM.

Detection of Human c‑Abl by Simple WesternTM.

Simple Western lane view shows lysates of Jurkat human acute T cell leukemia cell line, loaded at 0.2 mg/mL. A specific band was detected for c-Abl at approximately 149 kDa (as indicated) using 10 µg/mL of Goat Anti-Human c-Abl Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5414) followed by 1:50 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.

Applications for Human c‑Abl Antibody

Application
Recommended Usage

Simple Western

10 µg/mL
Sample: Jurkat human acute T cell leukemia cell line

Western Blot

1 µg/mL
Sample: Jurkat human acute T cell leukemia cell line, MCF-7 human breast cancer cell line, and MDA-MB-453 human breast cancer cell line

Reviewed Applications

Read 2 reviews rated 3 using AF5414 in the following applications:

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: c-Abl

c-Abl (Abelson murine leukemia viral homolog 1) is a cytosolic member of the ABL subfamily, protein tyrosine kinase family of enzymes. It is ubiquitously expressed, and participates in multiple processes such as cell migration, actin reorganization, DNA damage response and apoptosis. Human c-Abl (I-B) is 1149 amino acids (aa) in length. It is myristoylated on Gly2 and contains one SH3 domain (aa 80‑140), an SH2 domain (aa 146‑236), a protein kinase region (aa 261‑512), three NLS's (aa 620‑634; 726‑739; 778‑791), one DNA-binding region (aa 888‑988), an NES motif (aa 1109‑1119) and an actin F-binding domain (aa 1120‑1149). There is one alternate splice form (I-A) that contains a 26 aa substitution for the N-terminal 45 amino acids. In chronic myelogenous leukemia, c-Abl is fused to the Bcr gene product, resulting in the production of a constitutively active tyrosine kinase.

Long Name

Abelson Murine Leukemia Viral Oncogene Homolog 1

Alternate Names

ABL1, BCR-ABL1, bcr/abl, cAbl, JTK7

Entrez Gene IDs

25 (Human); 11350 (Mouse); 311860 (Rat)

Gene Symbol

ABL1

UniProt

NP_009297

Additional c-Abl Products

Product Documents for Human c‑Abl Antibody

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human c‑Abl Antibody

For research use only

Citations for Human c‑Abl Antibody

Customer Reviews for Human c‑Abl Antibody (2)

3 out of 5
2 Customer Ratings
5 Stars
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4 Stars
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2 Stars
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1 Stars
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  • Human c-Abl Antibody
    Name: Anonymous
    Application: Microarray
    Sample Tested: EDTA Plasma
    Species: Human
    Verified Customer | Posted 12/06/2019
    Antibody was printed on custom arrays and incubated with fluorescently labeled human EDTA plasma. There was no signal on the array.
    Human c‑Abl Antibody AF5414
  • Human c-Abl Antibody
    Name: Anonymous
    Application: ELISA
    Sample Tested: Serum and Plasma
    Species: Human
    Verified Customer | Posted 11/11/2018

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