Human CILP-1 N-Terminal Fragment Alexa Fluor® 647-conjugated Antibody

Catalog #: AF5504R Datasheet / COA / SDS

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AF5504R-100UG

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Human CILP-1 N-Terminal Fragment Alexa Fluor® 647-conjugated Antibody Summary

Species Reactivity

Human

Specificity

Detects human CILP‑1 N-Terminal Fragment in direct ELISAs and Western blots. In direct ELISAs, less than 1% cross-reactivity with recombinant human CILP-1 C-terminal peptide is observed.

Source

Polyclonal Goat IgG

Purification

Antigen Affinity-purified

Immunogen

Mouse myeloma cell line NS0-derived recombinant human CILPâ€‘1 N-Terminal Fragment
Arg22-Arg720
Accession # NP_003604

Formulation

Supplied 0.2mg/ml in 1X PBS with RDF1 and 0.09% Sodium Azide

Label

Alexa Fluor 647 (Excitation= 650 nm, Emission= 668 nm)

Applications

Recommended Concentration

Sample

Western Blot

Optimal dilution of this antibody should be experimentally determined.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

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Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied

Background: CILP-1

The CILP-1 (cartilage intermediate-layer protein 1) gene product is a 132 kDa (predicted) monomeric glycoprotein that is found in both hyaline and fibrocartilage. It is a precursor for two secreted, proteolytically generated products, a 90 kDa N-terminal CILP-1, and a 62 kDa C-terminal NTPPHase-homolog. The N-terminus is suggested to serve as both a matrix structural protein, and an IGF-I/TGF-beta 1 suppressor sequestration molecule. Human CILP-1 spans aa 22‑720 of the CILP-1 precursor. It contains one TSP-1 domain (aa 149‑201), a C2‑type Ig-like region (aa 309‑395) and six potential N-glycosylation sites. Over aa 1‑720 of the CILP-1 precursor, human CILP-1 shares 89% aa identity with mouse CILP‑1, and 42% aa identity with human CILP-2.

Long Name

Cartilage Intermediate Layer Protein 1

Entrez Gene IDs

8483 (Human); 214425 (Mouse); 315761 (Rat)

Alternate Names

cartilage intermediate layer protein 1 C1; cartilage intermediate layer protein 1 C2; cartilage intermediate layer protein 1; cartilage intermediate layer protein, nucleotide pyrophosphohydrolase; Cartilage intermediate-layer protein; CILP; CILP1; CILP-1; HsT18872