Human Complement Component C1r Antibody (269104)

Detection of Complement Component C1r by Western Blot

ElpB and ElpQ preferentially bind activated forms of C1r and C1s. (A) Extracts from untreated (“−”) or pronase-treated (“+”) 1 × 107 strain B31-e2 spirochetes that ectopically produce the indicated surface lipoproteins were separated by SDS/PAGE and transferred to polyvinylidene difluoride (PVDF) membranes. The filters were probed with purified C1 complex (Top), activated C1r enzyme (Middle) or C1r proenzyme (Bottom), and bound probe revealed by anti-C1r antibody, followed by HRP-conjugated anti-mouse antibody. Shown is a representative of three experiments. (B) Filters prepared identically to A were probed with purified C1 complex (Top), activated C1s enzyme (Middle) or C1s proenzyme (Bottom), and bound probe revealed by anti-C1s antibody, followed by HRP-conjugated anti-mouse antibody. Shown is a representative of three experiments. (C and D) Biosensors immobilized with GST-ElpB (Upper) or GST-ElpQ (Lower) were tested by SPR for binding to the indicated concentrations of the enzyme or proenzyme forms of C1r (C) or C1s (D). Injection series were each performed in triplicate. For both C and D, steady-state affinity fits were determined by T200 Biacore Evaluation software and KD values are reported in Table 1. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/35312359), licensed under a CC-BY license. Not internally tested by R&D Systems.