Key Product Details

Species Reactivity

Human

Applications

Western Blot, Immunoprecipitation

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2B Clone # 313011
View all Complement Factor B Primary Antibodies »

Product Specifications

Immunogen

Mouse myeloma cell line NS0-derived recombinant human Complement Factor B
Thr26-Leu764
Accession # P00751

Specificity

Detects human Complement Factor B in direct ELISAs and Western blots. Does not cross-react with recombinant human (rh) Factor D or rhFactor I.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2B

Applications for Human Complement Factor B Antibody

Application
Recommended Usage

Immunoprecipitation

25 µg/mL
Sample: Conditioned cell culture medium spiked with Recombinant Human Complement Factor B, see our available Western blot detection antibodies

Western Blot

1 µg/mL
Sample: Recombinant Human Complement Factor B under non-reducing conditions only

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Complement Factor B

Complement Factor B, also known as C3/C5 convertase, is a serine protease of the alternative pathway of the complement system. The amino acid sequence of human Complement Factor B is 85% identical to that of rat and mouse.

Alternate Names

BF, BFD, C3/C5 convertase, CFAB, CFB, GBG, H2-Bf, PBF2

Entrez Gene IDs

629 (Human); 14962 (Mouse); 294257 (Rat)

Gene Symbol

CFB

UniProt

P00751

Additional Complement Factor B Products

Product Documents for Human Complement Factor B Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

Download SDS

Product Specific Notices for Human Complement Factor B Antibody

For research use only

Citations for Human Complement Factor B Antibody

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FAQs

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