Human CXCL1/2/3/GRO Pan Specific Antibody (31716)

Chemotaxis Induced by CXCL2/GRO beta and Neutralization by Human CXCL1/2/3/GRO Antibody.

Recombinant Human CXCL2/GRO beta (Catalog # 176-GB) chemoattracts the BaF3 mouse pro‑B cell line transfected with human CXCR2 in a dose-dependent manner (orange line). The amount of cells that migrated through to the lower chemotaxis chamber was measured by Resazurin (Catalog # AR002). Chemotaxis elicited by Recombinant Human CXCL2/GRO beta (6 ng/mL) is neutralized (green line) by increasing concentrations of Human CXCL1/2/3/GRO Monoclonal Antibody (Catalog # MAB276). The ND₅₀ is typically 0.15-0.75 µg/mL.

Detection of CXCL1/2/3/GRO Pan Specific by Western Blot

miR-182 upregulated NSCLC cells promote osteoclastogenesis through enhancing IL-8 secretion. A TRAP staining analysis of the osteoclast differentiation of RAW264.7 cells after cultured in the indicated CM for 6 days. Arrowheads indicate mature, multi-nucleated osteoclasts. B Transcriptome-seq combined with Gene Ontology analysis revealed the enrichment of multiple signaling pathways in miR-182-overexpressing A549 cells. C Volcano plot showing differentially expressed genes in miR-182-overexpressing A549 cells compared with the negative control cells, with selected cytokines highlighted. D qPCR analysis of the mRNA expression of IL-8, IL-1, IL-12 and CXCL3 in miR-182-overexpressing A549 (left) or H1299 cells relative to controls (right). E, F Western blotting of IL-8, IL-1, IL-12, and CXCL3 levels in CM from miR-182-overexpressing (left) or H1299 cells (right) relative to controls, with total protein staining (Coomassie) confirmed equal loading. F, G ELISA analysis of IL-8, IL-1, IL-12, and CXCL3 levels in the CM from miR-182-overexpressing (top) or H1299 cells (bottom) relative to controls (F) or in blood samples collected from mice bearing bone metastases developed by miR-182-overexpressing A549 cells or NC A549 cells (G). (H) TRAP staining analysis of the effects of depletion of indicated factors in the miR-182-CM (top) or their addition to the CM from A549 cells (bottom) on osteoclast differentiation of RAW264.7 cells. Arrowheads indicate mature, multi-nucleated osteoclasts. Scale bars, 50 μm. The average values ± SEM of three separate experiments are plotted. n.s., not significant; *P < 0.05; **P < 0.01; ***P < 0.001. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/37127752), licensed under a CC-BY license. Not internally tested by R&D Systems.

Detection of CXCL1/2/3/GRO Pan Specific by Western Blot

miR-182 upregulated NSCLC cells promote osteoclastogenesis through enhancing IL-8 secretion. A TRAP staining analysis of the osteoclast differentiation of RAW264.7 cells after cultured in the indicated CM for 6 days. Arrowheads indicate mature, multi-nucleated osteoclasts. B Transcriptome-seq combined with Gene Ontology analysis revealed the enrichment of multiple signaling pathways in miR-182-overexpressing A549 cells. C Volcano plot showing differentially expressed genes in miR-182-overexpressing A549 cells compared with the negative control cells, with selected cytokines highlighted. D qPCR analysis of the mRNA expression of IL-8, IL-1, IL-12 and CXCL3 in miR-182-overexpressing A549 (left) or H1299 cells relative to controls (right). E, F Western blotting of IL-8, IL-1, IL-12, and CXCL3 levels in CM from miR-182-overexpressing (left) or H1299 cells (right) relative to controls, with total protein staining (Coomassie) confirmed equal loading. F, G ELISA analysis of IL-8, IL-1, IL-12, and CXCL3 levels in the CM from miR-182-overexpressing (top) or H1299 cells (bottom) relative to controls (F) or in blood samples collected from mice bearing bone metastases developed by miR-182-overexpressing A549 cells or NC A549 cells (G). (H) TRAP staining analysis of the effects of depletion of indicated factors in the miR-182-CM (top) or their addition to the CM from A549 cells (bottom) on osteoclast differentiation of RAW264.7 cells. Arrowheads indicate mature, multi-nucleated osteoclasts. Scale bars, 50 μm. The average values ± SEM of three separate experiments are plotted. n.s., not significant; *P < 0.05; **P < 0.01; ***P < 0.001. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/37127752), licensed under a CC-BY license. Not internally tested by R&D Systems.