Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Validated:

Western Blot, Simple Western

Cited:

Simple Western

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # 946002
View all CYP3A4 Primary Antibodies »

Product Specifications

Immunogen

Human CYP3A4 synthetic peptide
Lys67-Pro80
Accession # P08684

Specificity

Detects human CYP3A4 in Western blots.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images for Human CYP3A4 Antibody

Detection of Human CYP3A4 antibody by Western Blot.

Detection of Human CYP3A4 by Western Blot.

Western blot shows lysate of human liver tissue. PVDF membrane was probed with 2 µg/mL of Mouse Anti-Human CYP3A4 Monoclonal Antibody (Catalog # MAB9079) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for CYP3A4 at approximately 50 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Detection of Human CYP3A4 by Simple WesternTM.

Simple Western lane view shows lysates of Human Liver, loaded at 0.2 mg/mL. A specific band was detected for CYP3A4 at approximately 55 kDa (as indicated) using 20 µg/mL of Mouse Anti-Human CYP3A4 Monoclonal Antibody (Catalog # MAB9079). This experiment was conducted under reducing conditions and using the 12-230kDa separation system.

Applications for Human CYP3A4 Antibody

Application
Recommended Usage

Simple Western

20 µg/mL
Sample: Human Liver

Western Blot

2 µg/mL
Sample: Human liver tissue

Reviewed Applications

Read 1 review rated 5 using MAB9079 in the following applications:

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: CYP3A4

CYP3A4 (cytochrome P450, subfamily 3A, polypeptide 4) belongs to the cytochrome P450 superfamily of monooxygenases that catalyze reactions involved in both drug metabolism and the synthesis of cholesterol, steroids, and other lipid components.  CYP3A4 is predominantly found in liver microsomes, but has also been detected in prostate, small and large intestine, bile duct, nasal mucosa, kidney and adrenal cortex.  It is an extremely important enzyme for drug metabolism; CYP3A4 catalyzes phase I oxidation reactions in an estimated 60% of all clinically used drugs, promoting in most cases their clearance by excretion.  CYP3A4 levels are induced by glucocorticoids and various xenobiotics, including drugs, pesticides and carcinogens.  Human CYP3A4 is a 50 kDa single-pass membrane protein, 503 amino acids in length.

Long Name

Cytochrome P450 3A4

Alternate Names

Albendazole Monooxygenase, Albendazole Sulfoxidase, CP33, CP34, CYP3A3, CYPIIIA4, NF-25, P450C3, P450PCN1, Quinine 3-Monooxygenase, Taurochenodeoxycholate 6-Alpha-Hydroxylase

Entrez Gene IDs

1576 (Human)

Gene Symbol

CYP3A4

UniProt

P08684

Additional CYP3A4 Products

Product Documents for Human CYP3A4 Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human CYP3A4 Antibody

For research use only

Citations for Human CYP3A4 Antibody

Customer Reviews for Human CYP3A4 Antibody (1)

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  • Human CYP3A4 Antibody
    Name: Danxin Wang
    Application: Simple Western
    Sample Tested: Liver tissue
    Species: Human
    Verified Customer | Posted 09/25/2019
    Human CYP3A4 Antibody MAB9079

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