Human DAPK3/ZIPK Antibody (729830)

R&D Systems | Catalog # MAB5290

R&D Systems

Key Product Details

Species Reactivity

Human

Applications

Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2B Clone # 729830
View all DAPK3/ZIPK Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant human DAPK3/ZIPK
Arg347-Arg454
Accession # O43293

Specificity

Detects human DAPK3/ZIPK in direct ELISAs. In direct ELISAs, no cross-reactivity with recombinant human (rh) DAPK1, rhDAPK2, rhDRAK1, or rhDRAK2 is observed.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2B

Scientific Data Images for Human DAPK3/ZIPK Antibody (729830)

Detection of Human DAPK3/ZIPK antibody by Western Blot.

Detection of Human DAPK3/ZIPK by Western Blot.

Western blot shows lysates of A431 human epithelial carcinoma cell line, HeLa human cervical epithelial carcinoma cell line, and HepG2 human hepatocellular carcinoma cell line. PVDF membrane was probed with 0.5 µg/mL of Mouse Anti-Human DAPK3/ZIPK Monoclonal Antibody (Catalog # MAB5290) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for DAPK3/ZIPK at approximately 55 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Applications for Human DAPK3/ZIPK Antibody (729830)

Application
Recommended Usage

Western Blot

0.5 µg/mL
Sample: A431 human epithelial carcinoma cell line, HeLa human cervical epithelial carcinoma cell line, and HepG2 human hepatocellular carcinoma cell line

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Sterile PBS to a final concentration of 0.5 mg/mL. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: DAPK3/ZIPK

DAPK3 (Death-associated protein kinase 3; also ZIP kinase and DLK) is a 52 kDa member of the CAMK Ser/Thr kinase family, protein kinase superfamily of enzymes.  In general, mouse DAPK3 is nuclear while human DAPK3 is cytosolic.  DAPK3 is found in smooth muscle where it phosphorylates the regulatory light chain of myosin and stabilizes thick filament formation.  Human DAPK3 is 454 amino acids (aa) in length.  It contains one protein kinase domain (aa 134-275) and a C‑terminal Leu-zipper motif (aa 427-441) that mediates homo- and hetero-dimerization.  There is one 35 kDa alternate splice form that shows a 10 aa substitution for aa 313-454.  This form has both a nuclear and cytosolic localization. Over aa 347‑454, human DAPK3 is 65% aa identical to mouse DAPK3.   

Long Name

Death-associated Protein Kinase 3

Alternate Names

Dlk, ZIPK

Entrez Gene IDs

1613 (Human); 13144 (Mouse); 64391 (Rat)

Gene Symbol

DAPK3

UniProt

O43293 (Human)

Additional DAPK3/ZIPK Products

Product Documents for Human DAPK3/ZIPK Antibody (729830)

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human DAPK3/ZIPK Antibody (729830)

For research use only

Related Research Areas

Epigenetics

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