Human DcR3/TNFRSF6B Antibody (229812)

R&D Systems | Catalog # MAB2135

R&D Systems

Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Validated:

Western Blot, Neutralization

Cited:

Western Blot, Neutralization

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # 229812
View all DcR3/TNFRSF6B Primary Antibodies »

Product Specifications

Immunogen

Mouse myeloma cell line NS0-derived recombinant human DcR3/TNFRSF6B
Val24-His300
Accession # O95407

Specificity

Detects human DcR3/TNFRSF6B in direct ELISAs and Western blots. In Western blots, no cross-reactivity with recombinant human (rh) Fas, rhOPG, rhTNF R2, rhTRAIL R1, rhTRAIL R2, rhTRAIL R3, or rhTRAIL R4 is observed.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Endotoxin Level

<0.10 EU per 1 μg of the antibody by the LAL method.

Scientific Data Images for Human DcR3/TNFRSF6B Antibody (229812)

DcR3/TNFRSF6B Inhibition of Fas Ligand/TNFSF6-induced Apoptosis and Neutralization by Human DcR3/TNFRSF6B Antibody.

DcR3/TNFRSF6B Inhibition of Fas Ligand/TNFSF6-induced Apoptosis and Neutralization by Human DcR3/TNFRSF6B Antibody.

In the presence of a cross-linking antibody, Mouse polyHistidine Monoclonal Antibody (10 µg/mL, Catalog # MAB050), Recombinant Human DcR3/TNFRSF6B Fc Chimera (Catalog # 142-DC) inhibits Recombinant Human Fas Ligand/TNFSF6 (Catalog # 126-FL) induced apoptosis in the Jurkat human acute T cell leukemia cell line in a dose-dependent manner (orange line). Under these conditions, inhibition of Recombinant Human Fas Ligand/TNFSF6 (20 ng/mL) activity elicited by Recombinant Human DcR3/TNFRSF6B Fc Chimera (1 µg/mL) is neutralized (green line) by increasing concentrations of Mouse Anti-Human DcR3/TNFRSF6B Monoclonal Antibody (Catalog # MAB2135). The ND50 is typically 0.1-0.4 µg/mL.
Human DcR3 / TNFRSF6B ELISA Standard Curve

Human DcR3 / TNFRSF6B ELISA Standard Curve

Recombinant Human DcR3/TNFRSF6B Fc Chimera (Catalog # 142-DC) was serially diluted and captured by Mouse Anti-Human DcR3/TNFRSF6B Monoclonal Antibody (Catalog # MAB21351) coated on a Clear Polystyrene Microplate (Catalog # DY990). Mouse Anti-Human DcR3/TNFRSF6B Monoclonal Antibody (Catalog # MAB2135) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)

Applications for Human DcR3/TNFRSF6B Antibody (229812)

Application
Recommended Usage

Western Blot

1 µg/mL
Sample: Recombinant Human DcR3/TNFRSF6B Fc Chimera (Catalog # 142-DC)
under non-reducing conditions only

Neutralization

Measured by its ability to neutralize DcR3/TNFRSF6B-mediated inhibition of apoptosis in the Jurkat human acute T cell leukemia cell line. The Neutralization Dose (ND50) is typically 0.1-0.4 µg/mL in the presence of 1 µg/mL Recombinant Human DcR3/TNFRSF6B Fc Chimera, 20 ng/mL Recombinant Human Fas Ligand/TNFSF6, and 10 µg/mL of a cross-linking antibody, Mouse polyHistidine Monoclonal Antibody.

Reviewed Applications

Read 1 review rated 5 using MAB2135 in the following applications:

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: DcR3/TNFRSF6B

Human decoy receptor 3 (DcR3), also called TNFRSF6B, TR6 and M68, is a member of the TNF receptor superfamily. The cDNA of DcR3 encodes a 300 amino acid (aa) polypeptide with a putative 23 aa signal peptide. Like osteoprotegerin (OPG), DcR3 lacks a transmembrane sequence and is a secreted protein. DcR3 shares sequence identity with OPG (31%), TNFRII (29%) and Fas (17%). It was found to be expressed in a variety of different tissues and at high levels in many malignant tumors. Among TNF family members, DcR3 was shown to bind with Fas ligand (FasL) and LIGHT and inhibit FasL- and LIGHT-induced apoptosis. Thus, in addition to DcR1, DcR2 and OPG, DcR3 is another TNFR family molecule which modulates ligands that induce apoptosis. Overexpression of DcR3 might be a mechanism by which certain tumors escape immune-cytotoxic attack.

References

  1. Pitti, R.M. et al. (1998) Nature 396:699.
  2. Yu, K-Y. et al. (1999) J. Biochem. Chem. 274:13733.
  3. Bai, C. et al. (2000) Proc. Natl. Acad. Sci. USA. 97:1230.

Long Name

Decoy Receptor 3

Alternate Names

M68, TNFRSF6B, TR6

Entrez Gene IDs

8771 (Human); 107126607 (Cynomolgus Monkey)

Gene Symbol

TNFRSF6B

UniProt

O95407

Additional DcR3/TNFRSF6B Products

Product Documents for Human DcR3/TNFRSF6B Antibody (229812)

Certificate of Analysis

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Product Specific Notices for Human DcR3/TNFRSF6B Antibody (229812)

For research use only

Citations for Human DcR3/TNFRSF6B Antibody (229812)

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  • Human DcR3/TNFRSF6B Antibody
    Name: Anonymous
    Application: ELISA
    Sample Tested: Cell culture supernatant
    Species: Human
    Verified Customer | Posted 09/23/2016

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