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Streptavidin-HRP

R&D Systems | Catalog # DY998

Set of 5 vials enough for 75 plates total
R&D Systems
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Key Product Details

Applications

ELISA

Product Summary for Streptavidin-HRP

INTENDED USE & DESCRIPTION

Streptavidin-HRP (Part # 890803) contains 5 vials (1 mL/vial) of streptavidin conjugated to horseradish peroxidase for use with biotinylated detection antibodies in ELISAs and Western Blotting.

STORAGE & STABILITY

Store unopened at 2-8 °C. Stable for up to 6 months after opening when stored at 2-8 °C. Do Not Freeze.

REAGENT PREPARATION

For use in ELISAs, dilute to the working concentration specified on the vial label using 1% BSA/PBS or Reagent Diluent Concentrate 2 (Catalog # DY995) diluted 1:10 in deionized water. For use in Western blots, the suggested dilution is 1:200-1:2000 in an appropriate diluent. Use the diluted reagent within 60 minutes. 

Background: Streptavidin

Streptavidin is a tetrameric protein purified from Streptomyces sp. that binds very tightly to the vitamin biotin with a Kd of ~ 10-14 mol/l. The high affinity recognition of biotin and biotinylated molecules has made streptavidin one of the most important components in diagnostics and laboratory kits.

Alternate Names

SA V1; SA V2; Streptavidin V1; Streptavidin V2

Additional Streptavidin Products

Product Documents for Streptavidin-HRP

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Streptavidin-HRP

For research use only

Citations for Streptavidin-HRP

Customer Reviews for Streptavidin-HRP (26)

4.9 out of 5
26 Customer Ratings
5 Stars
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4 Stars
12%
3 Stars
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  • Streptavidin-HRP
    Name: Anonymous
    Verified Customer | Posted 02/18/2025
    Streptavidin-HRP DY998
  • Streptavidin-HRP
    Name: Anonymous
    Verified Customer | Posted 11/08/2023
    Used it for mouse serum and plasma samples and worked fine. I have used 1.5 hrs of incubation for both the samples.
    Streptavidin-HRP DY998
  • Streptavidin-HRP
    Name: Anonymous
    Verified Customer | Posted 07/24/2023
    I used it for human serum samples at 1:50000 dilution and singaps were great.
    Streptavidin-HRP DY998
  • Streptavidin-HRP
    Name: Anonymous
    Verified Customer | Posted 06/23/2023
    Extracelullar vesicles derived from conditioned media were subjected to CD63 determination by a home-made ELISA. Elution profile after Ion exchange chropmatography
    Streptavidin-HRP DY998
  • Streptavidin-HRP
    Name: Cassie Berta
    Verified Customer | Posted 05/09/2023
    Great HRP use for an ELISA assay. Gave me a great consistent standard curve for a residual assay.
    Streptavidin-HRP DY998
  • Streptavidin-HRP
    Name: Dilanka Ratnayake
    Verified Customer | Posted 01/17/2023
  • Streptavidin-HRP
    Name: Anonymous
    Verified Customer | Posted 11/02/2022
    GOOD SIGNAL FOR ELISA ASSAY.
    Streptavidin-HRP DY998
  • Streptavidin-HRP
    Name: Rob Knight
    Verified Customer | Posted 12/09/2021
    Direct ELISA of permeabilized or intact small extracellular vesicles
    Streptavidin-HRP DY998
  • Streptavidin-HRP
    Name: Anonymous
    Verified Customer | Posted 11/18/2021
    Worked great for ELISA assay. Provides acceptable signal to noise ratio consistently.
    Streptavidin-HRP DY998
  • Streptavidin-HRP
    Name: Anonymous
    Verified Customer | Posted 09/28/2021
  • Streptavidin-HRP
    Name: Ricardo Malvicini
    Verified Customer | Posted 09/10/2021
    Semi-quantification of CD63 by ELISA, in different fractions of ion exchange chromatography isolated EV.
    Streptavidin-HRP DY998
  • Streptavidin-HRP
    Name: Anonymous
    Verified Customer | Posted 05/13/2021
  • Streptavidin-HRP
    Name: Anonymous
    Verified Customer | Posted 10/23/2020
  • Streptavidin-HRP
    Name: Josh Richards
    Verified Customer | Posted 02/26/2020
    Used Strep-HRP to develop an IL-25 ELISA. Works as expected at the suggested concentration. Very nice results, standards and control show up nicely.
    Streptavidin-HRP DY998
  • Streptavidin-HRP
    Name: Shubhankar Ambike
    Verified Customer | Posted 01/22/2020
    1:200 dilution in 1% milk used for wesern blot of biotinylated viral protein. 15% gel, wet transfer, overnight incubation with Streptavidin-HRP. Only specific bands were seen and no background at all. Highly recommended
    Streptavidin-HRP DY998
  • Streptavidin-HRP
    Name: Anonymous
    Verified Customer | Posted 11/06/2019
    Streptavidin-HRP DY998
  • Streptavidin-HRP
    Name: Anonymous
    Verified Customer | Posted 03/29/2019
    Used SA-HRP 1:200 for 30 min to compared a in-house biotinylated antibody to the unconjugated with direct ELISA
    Streptavidin-HRP DY998
  • Streptavidin-HRP
    Name: Anonymous
    Verified Customer | Posted 11/26/2018
  • Streptavidin-HRP
    Name: Ed Lo
    Verified Customer | Posted 11/06/2018
  • Streptavidin-HRP
    Name: Anonymous
    Verified Customer | Posted 08/17/2018
  • Streptavidin-HRP
    Name: Anonymous
    Verified Customer | Posted 04/16/2018
    We used this Ab for the Elisa in a 1:200 dilution, it works very well, showed no background.
  • Streptavidin-HRP
    Name: Balaji Mahender
    Verified Customer | Posted 12/18/2017
  • Streptavidin-HRP (set of 5 vials enough for 75 plates total)
    Name: Anonymous
    Verified Customer | Posted 12/05/2017
  • Streptavidin-HRP (set of 5 vials enough for 75 plates total)
    Name: Anonymous
    Verified Customer | Posted 07/20/2017
    Standard ELISA protocol observed for concentration analysis
    Streptavidin-HRP DY998
  • Streptavidin-HRP (set of 5 vials enough for 75 plates total)
    Name: Anonymous
    Verified Customer | Posted 12/21/2016
    Western blot of biotinated molecule.1:1000dilution of the product in BSA
  • Streptavidin-HRP (set of 5 vials enough for 75 plates total)
    Name: Anonymous
    Verified Customer | Posted 06/02/2016

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