Human Dkk-1 Antibody (141119)

R&D Systems | Catalog # MAB1096

R&D Systems

Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Validated:

Western Blot

Cited:

Western Blot, ELISA Development

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # 141119
View all Dkk-1 Primary Antibodies »

Product Specifications

Immunogen

S. frugiperda insect ovarian cell line Sf 21-derived recombinant human Dkk-1
Thr32-His266
Accession # O94907

Specificity

Detects human Dkk-1 in direct ELISAs and Western blots. In direct ELISAs and Western blots, this antibody shows approximately 50% cross‑reactivity with recombinant mouse (rm) Dkk-1 and no cross-reactivity with rmDkk-2, rhDkk-3, or rhDkk-4.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images for Human Dkk-1 Antibody (141119)

Detection of Dkk-1 by Immunohistochemistry

Detection of Dkk-1 by Immunohistochemistry

In vivo experiments confirmed that tumor-derived NOX4 inhibit the apoptosis of GC cells by recruiting neutrophils into the tumor microenvironment through GM-CSF. (A) Tumor formation and treatment model of NOD/SCID mice. (B-C) NOD/SCID mice (6 per group) were inoculated subcutaneously with NOX4-overexpression MKN45 GC cell. Once tumor size reached 2mm3, 5×106/200μL of neutrophils from healthy donors and pre-treated with different chemokine neutralizing antibodies (anti-CD14, anti-GM-CSF and anti-DKK1, respectively) were injected into the tail vein. Tumor growth curves (C) and tumor burdens (D) plotted from data measured every 4 days. Data points are shown only up to the time point at which the average tumor volume in any group first reached the predefined ethical limit. Mice were euthanized thereafter. (E) Neutrophils infiltration in tumors determined by CD66b IHC in each group. The red arrow refers to neutrophils. (F) Apoptosis of gastric cancer cells determined by Tunel Apoptosis Detection Kit in each group. Data are presented as the mean ± SD. *P < 0.05; **P < 0.01; ***P< 0.001. Image collected and cropped by CiteAb from the following open publication (https://www.ijbs.com/v22p4919.htm), licensed under a CC-BY license. Not internally tested by R&D Systems.

Applications for Human Dkk-1 Antibody (141119)

Application
Recommended Usage

Western Blot

1 µg/mL
Sample: Recombinant Human Dkk‑1 (Catalog # 5439-DK)
under non-reducing conditions only

Reviewed Applications

Read 1 review rated 5 using MAB1096 in the following applications:

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Dkk-1

Dkk-1 is a Dickkopf-related protein that inhibits Wnt signaling by binding the Wnt co-receptor LRP5/6. It also binds Kremen-1 and Kremen-2. Dkk-1 is important in embryonic development.

Long Name

Dickkopf-1

Alternate Names

Dkk1

Entrez Gene IDs

22943 (Human); 13380 (Mouse); 102123735 (Cynomolgus Monkey)

Gene Symbol

DKK1

UniProt

O94907

Additional Dkk-1 Products

Product Documents for Human Dkk-1 Antibody (141119)

Certificate of Analysis

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Product Specific Notices for Human Dkk-1 Antibody (141119)

For research use only

Citations for Human Dkk-1 Antibody (141119)

Customer Reviews for Human Dkk-1 Antibody (141119) (1)

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  • Human Dkk-1 Antibody
    Name: Anonymous
    Application: Western Blot
    Sample Tested: DKK-1 overexpressed HEK293 cells
    Species: Human
    Verified Customer | Posted 08/25/2021
    1:1000 dilution.
    Human Dkk-1 Antibody (141119) MAB1096

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