Human DOK3 Antibody (642316)

R&D Systems | Catalog # MAB4795

R&D Systems

Key Product Details

Species Reactivity

Human

Applications

Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2B Clone # 642316
View all DOK3 Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant human DOK3
Gly181-Lys271
Accession # Q7L591

Specificity

Detects human DOK3 in direct ELISAs and Western blots.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2B

Scientific Data Images for Human DOK3 Antibody (642316)

Detection of Human DOK3 antibody by Western Blot.

Detection of Human DOK3 by Western Blot.

Western blot shows lysates of human peripheral blood mononuclear cells (PBMC). PVDF Membrane was probed with 0.25 µg/mL of Mouse Anti-Human DOK3 Monoclonal Antibody (Catalog # MAB4795) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for DOK3 at approximately 65 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 2.

Applications for Human DOK3 Antibody (642316)

Application
Recommended Usage

Western Blot

0.25 µg/mL
Sample: Human peripheral blood mononuclear cells (PBMC)

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: DOK3

DOK3 (downstream of kinase 3) is a 53 kDa (predicted) member of the DOK family of cytoplasmic adaptor proteins and is expressed in B cells and macrophages.  Human DOK3 cDNA encodes 496 amino acids (aa) including one pleckstrin homology region (aa 63‑179) and an IRS-type phosphotyrosine binding domain (PTB) (aa 213‑317). Alternate splicing generates 330, 228 and 216 aa isoforms with N-terminal and C-terminal truncations and substitutions. Human DOK3 shares approximately 80% aa sequence identity with mouse and rat DOK3 within aa 181‑271, which are common to all four isoforms. Phosphorylation of tyrosine residues in DOK3 regulates its interaction with SH2 and SH3 containing proteins, leading to inhibition of signaling from immunoreceptors.

Long Name

Downstream of Tyrosine Kinase 3

Alternate Names

docking protein 3, DOKL, Dok-like protein, Downstream of tyrosine kinase 3, FLJ22570, FLJ39939

Entrez Gene IDs

79930 (Human); 27261 (Mouse); 306760 (Rat)

Gene Symbol

DOK3

UniProt

Q7L591 (Human)

Additional DOK3 Products

Product Documents for Human DOK3 Antibody (642316)

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human DOK3 Antibody (642316)

For research use only

Citations for Human DOK3 Antibody (642316)

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