DPP10 (dipeptidyl peptidase 10; also DPPY, DPRP3 and DPL2) is a 91‑97 kDa member of the peptidase S9B family. It is a type II transmembrane glycoprotein principally expressed in neurons and T cells. Although it is a peptidase member, it has no catalytic activity. Instead, it assists in the membrane trafficking and functioning of Kv4 K+ channels. Human DPP10 is 796 amino acids (aa) in length. It contains a 34 aa cytoplasmic N-terminus and a 741 aa extracellular domain (ECD) (aa 56‑796). Three potential isoforms exist, all involving aa substitutions limited to the first 20 amino acids. The ECD of human DPP10 shares 89% aa sequence identity with mouse DPP10 ECD.
Key Product Details
Species Reactivity
Human
Applications
Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Sheep IgG
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Product Specifications
Immunogen
S. frugiperda insect ovarian cell line Sf 21-derived recombinant human DPP10
Leu56-Glu796 (Pro340Ala)
Accession # NP_065919
Leu56-Glu796 (Pro340Ala)
Accession # NP_065919
Specificity
Detects human DPP10 in direct ELISAs and Western blots. In direct ELISAs and Western blots, less than 5% cross‑reactivity with recombinant human (rh) DPP6 and rhDPP4 is observed.
Clonality
Polyclonal
Host
Sheep
Isotype
IgG
Applications for Human DPP10 Antibody
Application
Recommended Usage
Western Blot
0.1 µg/mL
Sample: Recombinant Human DPP10
Sample: Recombinant Human DPP10
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
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Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Calculators
Background: DPP10
Long Name
Dipeptidyl-peptidase 10
Alternate Names
DPL2, DPPY, DPRP3
Gene Symbol
DPP10
UniProt
Additional DPP10 Products
Product Documents for Human DPP10 Antibody
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Note: Certificate of Analysis not available for kit components.
Product Specific Notices for Human DPP10 Antibody
For research use only
Related Research Areas
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- Cellular Response to Hypoxia Protocols
- R&D Systems Quality Control Western Blot Protocol
- Troubleshooting Guide: Western Blot Figures
- Western Blot Conditions
- Western Blot Protocol
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- Western Blot Troubleshooting
- Western Blot Troubleshooting Guide
- View all Protocols, Troubleshooting, Illustrated assays and Webinars