Human E2F-4 Alexa Fluor® 594-conjugated Antibody

Catalog #: AF5139T Datasheet / COA / SDS

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AF5139T-100UG

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All E2F-4 Antibodies

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Human E2F-4 Alexa Fluor® 594-conjugated Antibody Summary

Species Reactivity

Human

Specificity

Detects endogenous human E2F-4 in Western bots.

Source

Polyclonal Goat IgG

Purification

Antigen Affinity-purified

Immunogen

E. coli-derived recombinant human E2F-4
Lys178-Asp306
Accession # Q16254

Formulation

Supplied 0.2mg/ml in 1X PBS with RDF1 and 0.09% Sodium Azide

Label

Alexa Fluor 594 (Excitation= 590 nm, Emission= 617 nm)

Applications

Recommended Concentration

Sample

Western Blot

Optimal dilution of this antibody should be experimentally determined.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied

Background: E2F-4

E2F-4 (viral E2-associated factor 4) is a 50 kDa member of the E2F/DP family of transcription factors. It is ubiquitously and constitutively expressed, and forms a DNA-binding E2F heterodimeric complex with DP-1. This complex, when associated with p130/RBL2 and a series of LIN-numbered proteins, represses cell cycle‑dependent genes during G0 and G1. Human E2F-4 is 413 amino acids (aa) in length and contains a poly-Pro region (aa 9‑12), a DNA-binding domain (aa 16‑85), a dimerization segment (aa 86‑181), poly-Ser region (aa 307‑327), and an RBL1/RBL2 binding domain (aa 390‑407). There is one potential alternate start site at Met261. Over aa 178‑306, human E2F-4 shares 80% and 83% aa identity with mouse and canine E2F-4, respectively.

Long Name

E2F Transcription Factor 4

Entrez Gene IDs

1874 (Human); 104394 (Mouse)

Alternate Names

E2F transcription factor 4, p107/p130-binding; E2F4; E2F-4; E2F-4p107/p130-binding protein; transcription factor E2F4