Human EDA2R/TNFRSF27/XEDAR Antibody

R&D Systems | Catalog # AF1093

R&D Systems

Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Validated:

Western Blot, Blockade of Receptor-ligand Interaction

Cited:

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG
View all EDA2R/TNFRSF27/XEDAR Primary Antibodies »

Product Specifications

Immunogen

Mouse myeloma cell line NS0-derived recombinant human EDA2R/TNFRSF27/XEDAR
Met1-Glu136
Accession # Q9HAV5

Specificity

Detects human EDA2R/TNFRSF27/XEDAR in direct ELISAs and Western blots.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Endotoxin Level

<0.10 EU per 1 μg of the antibody by the LAL method.

Scientific Data Images for Human EDA2R/TNFRSF27/XEDAR Antibody

Human EDA2R / TNFRSF27 / XEDAR ELISA Standard Curve

Human EDA2R / TNFRSF27 / XEDAR ELISA Standard Curve

Recombinant Human EDA2R/TNFRSF27/XEDAR Fc Chimera (Catalog # 1093-XD) was serially diluted and captured by Mouse Anti-Human EDA2R/TNFRSF27/XEDAR Monoclonal Antibody (Catalog # MAB10931) coated on a Clear Polystyrene Microplate (Catalog # DY990). Goat Anti-Human EDA2R/TNFRSF27/XEDAR Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1093) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)

Applications for Human EDA2R/TNFRSF27/XEDAR Antibody

Application
Recommended Usage

Blockade of Receptor-ligand Interaction

In a functional ELISA, 1-4 µg/mL of this antibody will block 50% of the binding of 5 ng/mL of Recombinant Human EDA‑A2/Ectodysplasin A2 (Catalog # 922-ED) to immobilized Recombinant Human EDA2R/TNFRSF27/XEDAR Fc Chimera (Catalog # 1093-XD) coated at 1 µg/mL (100 µL/well). At 10 μg/mL, this antibody will block >90% of the binding.

Western Blot

0.1 µg/mL
Sample: Recombinant Human EDA2R/TNFRSF27/XEDAR Fc Chimera (Catalog # 1093-XD)

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: EDA2R/TNFRSF27/XEDAR

X-linked ectodysplasin receptor (XEDAR) is a type III transmembrane that lacks an N-terminal signal peptide. It is an X-linked member of the TNF Receptor Superfamily (TNFRSF). Human XEDAR is a 297 amino acid (aa) protein with a 136 aa extracellular domain, a 21 aa transmembrane domain, and a 140 aa cytoplasmic domain. Within the TNFRSF, XEDAR shares the highest homologies with EDAR and TNFRSF19/TROY. EDA-A2 is the XEDAR ligand. XEDAR expression is principally found in embryonic hair follicles. XEDAR, EDAR, EDA-A1 and EDA-A2 have been associated with hypohidrotic ectodermal dysplasia (HED). HED is characterized by abnormalities in hair, teeth, and eccrine sweat gland morphogenesis. HED was initially found to associate with two gene loci, tabby and downless. Tabby was later identified as the gene for EDA and downless as the autosomal EDAR gene. EDA has two splice variants, EDA-A1 and EDA-A2 which differ by only two amino acids. Despite this minor difference, the EDA isoforms display strong receptor specificity. EDA-A1 only binds EDAR, whereas EDA-A2 only binds to XEDAR. Mutations in EDA, EDAR and XEDAR have been associated with HED.

References

  1. Headon, D.J. and P.A Overbeek (1999) Nat. Genet. 22:370.
  2. Kumar, A. et al. (2000) J. Biol. Chem. 276:2668.
  3. Monreal, A.W. et al. (1999) Nat. Genet. 22:366.
  4. Schneider, P. et al. (2001) J. Biol. Chem. 276:18819.
  5. Srivastava, A.K. et al. (1997) Proc. Natl. Acad. Sci. USA 94:13069.
  6. Yan, M. et al. (2000) Science 290:523.

Long Name

Ectodysplasin A2 Receptor

Alternate Names

EDAA2R, TNFRSF27, XEDAR

Entrez Gene IDs

60401 (Human); 245527 (Mouse); 710244 (Rhesus Macaque)

Gene Symbol

EDA2R

UniProt

Q9HAV5

Additional EDA2R/TNFRSF27/XEDAR Products

Product Documents for Human EDA2R/TNFRSF27/XEDAR Antibody

Certificate of Analysis

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Product Specific Notices for Human EDA2R/TNFRSF27/XEDAR Antibody

For research use only

Related Research Areas

NFkB Pathway
p53 Pathway

Citations for Human EDA2R/TNFRSF27/XEDAR Antibody

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