Human eIF2 alpha Alexa Fluor® 532-conjugated Antibody

Catalog #: AF3997X Datasheet / COA / SDS

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AF3997X-100UG

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All eIF2 alpha Antibodies

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Human eIF2 alpha Alexa Fluor® 532-conjugated Antibody Summary

Species Reactivity

Human

Specificity

Detects human, mouse, and rat eIF2 alpha in Western blots.

Source

Polyclonal Goat IgG

Purification

Antigen Affinity-purified

Immunogen

E. coli-derived recombinant human eIF2Î±
Met1-Asp315
Accession # Q53XC0

Formulation

Supplied 0.2mg/ml in 1X PBS with RDF1 and 0.09% Sodium Azide

Label

Alexa Fluor 532 (Excitation= 534 nm, Emission= 553 nm)

Applications

Recommended Concentration

Sample

Western Blot

Optimal dilution of this antibody should be experimentally determined.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied

Background: eIF2 alpha

elF2 alpha (eukaryotic translation initiation factor 2 alpha subunit) is a subunit of the elF2 protein, an important regulator of translation initiation. Phosphorylation of elF2 alpha on Ser 52 increases the affinity of elF2 alpha for elF2B, a guanine nucleotide exchange factor needed for the recycling of elF2-GDP to elF2-GTP. Reduction of elF2-GTP levels leads to the suppression of the overall rate of protein synthesis. Heme-regulated inhibitor (HRI), ER-resident protein kinase PERK, dsRNA activated protein kinase PKR, and the homologue of the S. cerevisiae protein kinase GCN2 are all elF2 alpha kinases.

Long Name

Eukaryotic Translation Initiation Factor 2 Alpha

Entrez Gene IDs

1965 (Human); 13665 (Mouse); 54318 (Rat)

Alternate Names

eIF2 alpha; EIF2; EIF-2; EIF2A; EIF-2A; EIF-2alpha; EIF2S1 eukaryotic translation initiation factor 2, subunit 1 alpha, 35kDa; EIF2S1