Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human, Mouse

Applications

Validated:

Western Blot, Simple Western

Cited:

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG
View all eIF2 alpha Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant human eIF2 alpha
Met1-Asp315
Accession # Q53XC0

Specificity

Detects human, mouse, and rat eIF2 alpha in Western blots.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for Human eIF2 alpha Antibody

Detection of Human eIF2a antibody by Western Blot.

Detection of Human eIF2 alpha by Western Blot.

Western blot shows lysates of K562 human chronic myelogenous leukemia cell line, MCF-7 human breast cancer cell line, CTLL-2 mouse cytotoxic T cell line, and NRK rat normal kidney cell line. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human eIF2a Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3997) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). A specific band was detected for eIF2a at approximately 38 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Detection of Human eIF2a antibody by Simple WesternTM.

Detection of Human eIF2 alpha by Simple WesternTM.

Simple Western lane view shows lysates of MCF-7 human breast cancer cell line, loaded at 0.2 mg/mL. A specific band was detected for eIF2a at approximately 42 kDa (as indicated) using 10 µg/mL of Goat Anti-Human eIF2a Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3997) followed by 1:50 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system. Non-specific interaction with the 230 kDa Simple Western standard may be seen with this antibody.

Applications for Human eIF2 alpha Antibody

Application
Recommended Usage

Simple Western

10 µg/mL
Sample: MCF‑7 human breast cancer cell line

Western Blot

1 µg/mL
Sample: K562 human chronic myelogenous leukemia cell line chronic myelogenous leukemia, MCF-7 human breast cancer cell liner, CTLL-2 mouse cytotoxic T cell line, and NRK rat normal kidney cells

Reviewed Applications

Read 2 reviews rated 5 using AF3997 in the following applications:

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: eIF2 alpha

elF2 alpha (eukaryotic translation initiation factor 2 alpha subunit) is a subunit of the elF2 protein, an important regulator of translation initiation. Phosphorylation of elF2 alpha on Ser 52 increases the affinity of elF2 alpha for elF2B, a guanine nucleotide exchange factor needed for the recycling of elF2-GDP to elF2-GTP. Reduction of elF2-GTP levels leads to the suppression of the overall rate of protein synthesis. Heme-regulated inhibitor (HRI), ER-resident protein kinase PERK, dsRNA activated protein kinase PKR, and the homologue of the S. cerevisiae protein kinase GCN2 are all elF2 alpha kinases.

Long Name

Eukaryotic Translation Initiation Factor 2 Alpha

Alternate Names

EIF2S1

Entrez Gene IDs

1965 (Human); 13665 (Mouse); 54318 (Rat)

Gene Symbol

EIF2S1

UniProt

Q53XC0

Additional eIF2 alpha Products

Product Documents for Human eIF2 alpha Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human eIF2 alpha Antibody

For research use only

Related Research Areas

Translation Regulation

Citations for Human eIF2 alpha Antibody

Customer Reviews for Human eIF2 alpha Antibody (2)

5 out of 5
2 Customer Ratings
5 Stars
100%
4 Stars
0%
3 Stars
0%
2 Stars
0%
1 Stars
0%

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Customer Images

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  • Human eIF2 alpha Antibody
    Name: Jothi Jayavel
    Application: Western Blot
    Sample Tested: MDA-MB-231 human breast cancer cell line
    Species: Human
    Verified Customer | Posted 01/11/2018
    Human eIF2 alpha Antibody AF3997
  • Human eIF2 alpha Antibody
    Name: Dharmaraja Allimuthu
    Application: Western Blot
    Sample Tested: Embryonic stem cells
    Species: Human
    Verified Customer | Posted 12/27/2017
    Human eIF2 alpha Antibody AF3997

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