Key Product Details

Species Reactivity

Human

Applications

Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Rat IgG2B Clone # 430516
View all eIF2 alpha Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant human eIF2 alpha
Met1-Asp315
Accession # Q53XC0

Specificity

Detects human eIF2 alpha in Western blots.

Clonality

Monoclonal

Host

Rat

Isotype

IgG2B

Scientific Data Images for Human eIF2 alpha Antibody

Detection of Human eIF2a antibody by Western Blot.

Detection of Human eIF2 alpha by Western Blot.

Western blot shows lysates of Jurkat human acute T cell leukemia cell line, K562 human chronic myelogenous leukemia cell line, and MCF-7 human breast cancer cell line. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human eIF2a Monoclonal Antibody (Catalog # MAB3997) followed by HRP-conjugated Anti-Rat IgG Secondary Antibody (Catalog # HAF005). A specific band was detected for eIF2a at approximately 40 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Applications for Human eIF2 alpha Antibody

Application
Recommended Usage

Western Blot

1 µg/mL
Sample: Jurkat human acute T cell leukemia cell line, K562 human chronic myelogenous leukemia cell line, and MCF-7 human breast cancer cell line

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: eIF2 alpha

Eukaryotic translation initiation factor 2 alpha subunit (eIF2 alpha ) is a subunit of the eIF2 protein, an important regulator of translation initiation. Phosphorylation of eIF2 alpha on Ser 52 increases the affinity of eIF2 alpha for eIF2B, a guanine nucleotide exchange factor needed for the recycling of eIF2-GDP to eIF2-GTP. Reduction of eIF2-GTP levels leads to the suppression of the overall rate of protein synthesis. Heme-regulated inhibitor (HRI), ER-resident protein kinase PERK, dsRNA activated protein kinase PKR, and the homologue of the S. cerevisiae protein kinase GCN2 are all eIF2 alpha kinases.

Long Name

Eukaryotic Translation Initiation Factor 2 Alpha

Alternate Names

EIF2S1

Entrez Gene IDs

1965 (Human); 13665 (Mouse); 54318 (Rat)

Gene Symbol

EIF2S1

UniProt

Q53XC0

Additional eIF2 alpha Products

Product Documents for Human eIF2 alpha Antibody

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human eIF2 alpha Antibody

For research use only

Related Research Areas

Translation Regulation

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