Key Product Details

Species Reactivity

Rat

Applications

Control

Label

Unconjugated

Antibody Source

Monoclonal Rat IgG2B Clone # 141945
View all IgG2B Isotype Controls »

Product Specifications

Immunogen

KLH

Specificity

Selected for use as a rat IgG2B isotype control. In direct ELISAs, does not cross-react with 102 different cytokines.

Clonality

Monoclonal

Host

Rat

Isotype

IgG2B

Scientific Data Images for Rat IgG2B Isotype Control

Detection of Rat IgG Isotype Control by Flow Cytometry

Detection of Rat IgG Control by Flow Cytometry

Human peripheral blood mononuclear cells (PBMCs) were stained with Rat Anti-Mouse CD44 Monoclonal Antibody (Catalog # MAB6127, filled histogram) or Rat IgG Isotype Control Antibody (Catalog #MAB0061, open histogram), followed by Phycoerythrin-conjugated Anti-Rat IgG Secondary Antibody (Catalog F0105B).

Applications for Rat IgG2B Isotype Control

Application
Recommended Usage

Control

Negative control for use in conjunction with R&D Systems antibodies. Use at the same concentration as the detection antibody.

Reviewed Applications

Read 6 reviews rated 4.2 using MAB0061 in the following applications:

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: IgG2B

R&D Systems offers a range of secondary antibodies and controls for flow cytometry, immunohistochemistry, and Western blotting. We provide species-specific secondary antibodies that are available with a variety of conjugated labels.

Our NorthernLights fluorescent secondary antibodies are bright and resistant to photobleaching. We are currently offering secondary antibodies recognizing mouse, rat, goat, sheep, and rabbit IgG as well as chicken IgY. These reagents are available with three distinct excitation and emission maxima, making them ideal for multi-color fluorescence microscopy.

Long Name

Immunoglobulin G2B Fc

Additional IgG2B Products

Product Documents for Rat IgG2B Isotype Control

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

Download SDS

Product Specific Notices for Rat IgG2B Isotype Control

For research use only

Citations for Rat IgG2B Isotype Control

Customer Reviews for Rat IgG2B Isotype Control (6)

4.2 out of 5
6 Customer Ratings
5 Stars
33%
4 Stars
50%
3 Stars
17%
2 Stars
0%
1 Stars
0%

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Customer Images

Showing  1 - 5 的 6 reviews Showing All
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  • Rat IgG2B Isotype Control
    Name: Abby Sukarto
    Application: Flow Cytometry
    Sample Tested: Bone Extracts
    Species: Human
    Verified Customer | Posted 02/13/2020
    Rat IgG2B Isotype Control MAB0061
  • Rat IgG2B Isotype Control
    Name: Anonymous
    Application: Gel Shift
    Sample Tested: Purified protein
    Species: Rat
    Verified Customer | Posted 09/28/2019
    Rat IgG2B Isotype Control MAB0061
  • Rat IgG2B Isotype Control
    Name: Abby Sukarto
    Sample Tested: bone marrow and Bone Extracts
    Species: Human
    Verified Customer | Posted 09/10/2019
    Need optimization of your specific cell types.
  • Rat IgG2B Isotype Control
    Name: Ju Shi
    Application: Flow Cytometry
    Sample Tested: HEK293 human embryonic kidney cell line
    Species: Human
    Verified Customer | Posted 12/01/2017
  • Rat IgG2B Isotype Control
    Name: Anonymous
    Application: Flow Cytometry
    Sample Tested: Bone marrow cells
    Species: Mouse
    Verified Customer | Posted 09/01/2016
  • Rat IgG2B Isotype Control
    Name: Anonymous
    Application: Functional Assay
    Sample Tested: RAW 264.7 mouse monocyte/macrophage cell line
    Species: Mouse
    Verified Customer | Posted 07/11/2016
    72 hours cytotoxicity assay using secondary Zap conjugate
    Rat IgG2B Isotype Control MAB0061

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FAQs for Rat IgG2B Isotype Control

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    • A: The light chain is kappa. 
Showing  1 - 1 的 1 FAQ Showing All
View all FAQs for Isotype Controls