Enolase 2 (2-phospho-D-glycerate hydrolyase; also Neuron-specific Enolase, NSE, neural enolase and gamma-enolase) is a 46 kDa member of the Enolase family of enzymes. It is expressed in developing neurons and glia, is known to catalyze the generation of phosphoenolpyruvate, and is suggested to possess neurotrophic activity for neurons, likely through an extracellular mechanism. Human Enolase 2 is 434 amino acids (aa) in length. The enzymatic site spans most of the length of the molecule. Enolase 2 exists as both a noncovalently-linked homodimer, or heterodimer with alpha-enolase. Full-length human Enolase 2 shares 99% aa identity with both mouse and canine Enolase 2. It shares 83% aa identity with human enolases 1 and 3.
Human Enolase 2/Neuron-specific Enolase Quantikine ELISA Kit
R&D Systems | Catalog # DENL20
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Key Product Details
Assay Length
4.5 hours
Sample Type & Volume Required Per Well
Cell Culture Supernates (50 µL), Cell Lysates (50 µL), Serum (50 µL), Platelet-poor Heparin Plasma (50 µL)
Sensitivity
0.038 ng/mL
Assay Range
0.313-20 ng/mL (Cell Culture Supernates, Cell Lysates, Serum, Platelet-poor Heparin Plasma)
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Product Summary for Human Enolase 2/Neuron-specific Enolase Quantikine ELISA Kit
The Quantikine Human Enolase 2 Immunoassay is a 4.5 hour solid-phase ELISA designed to measure human Enolase 2 in cell culture supernates, cell lysates, serum, and plasma. It contains E. coli-expressed recombinant human Enolase 2 and has been shown to accurately quantitate the recombinant factor. Results obtained using natural human Enolase 2 showed linear curves that were parallel to the standard curves obtained using the Quantikine kit standards. These results indicate that this kit can be used to determine relative mass values for naturally occurring human Enolase 2.
Product Specifications
Assay Type
Solid Phase Sandwich ELISA
Format
96-well strip plate
Measurement
Quantitative ELISA
Detection Method
Colorimetric - 450nm (TMB)
Conjugate
HRP
Species
Human
Specificity
Natural and recombinant human Enolase 2
Cross-reactivity
< 0.5% cross-reactivity observed with available related molecules. Cross-species reactivity not tested.
Interference
No significant interference observed with available related molecules.
精度
Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision.
Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision.
Cell Culture Supernates, Cell Lysates, Platelet-poor Heparin Plasma, Serum
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 20 | 20 | 20 | 20 | 20 | 20 |
| Mean (pg/mL) | 2.15 | 8.41 | 13.0 | 1.96 | 8.50 | 12.6 |
| Standard Deviation | 0.03 | 0.17 | 0.36 | 0.13 | 0.33 | 0.55 |
| CV% | 1.4 | 2.0 | 2.8 | 6.7 | 3.9 | 4.3 |
Recovery for Human Enolase 2/Neuron-specific Enolase Quantikine ELISA Kit
The recovery of Enolase 2 spiked to levels throughout the range of the assay in various matrices was evaluated.
| Sample Type | Average % Recovery | Range % |
|---|---|---|
| Cell Culture Media (n=8) | 99 | 85-114 |
| Cell Lysates (n=8) | 105 | 85-115 |
| Platelet-poor Heparin Plasma (n=4) | 95 | 85-111 |
| Serum (n=4) | 95 | 85-110 |
Linearity
To assess the linearity of the assay, samples containing and/or spiked with high concentrations of Enolase 2 were serially diluted with Calibrator Diluent to produce samples with values within the dynamic range of the assay.
Scientific Data Images for Human Enolase 2/Neuron-specific Enolase Quantikine ELISA Kit
Human Enolase 2/Neuron-specific Enolase ELISA Standard Curve
Preparation and Storage
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.
Background: Enolase 2/Neuron-specific Enolase
Alternate Names
ENO2, gamma-Enolase, Neuronspecific Enolase, NSE
Gene Symbol
ENO2
Additional Enolase 2/Neuron-specific Enolase Products
Product Documents for Human Enolase 2/Neuron-specific Enolase Quantikine ELISA Kit
Product Specific Notices for Human Enolase 2/Neuron-specific Enolase Quantikine ELISA Kit
For research use only
⚠ WARNING: This product can expose you to chemicals including N,N-Dimethylforamide, which is known to the State of California to cause cancer. For more information, go to www.P65Warnings.ca.gov.Related Research Areas
Citations for Human Enolase 2/Neuron-specific Enolase Quantikine ELISA Kit
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Protocols
View specific protocols for Human Enolase 2/Neuron-specific Enolase Quantikine ELISA Kit (DENL20):
Refer to the product for complete assay procedure.
Bring all reagents and samples to room temperature before use. It is recommended that all samples, standards, and controls be assayed in duplicate.
- Prepare all reagents, standard dilutions, and samples as directed in the product insert.
- Remove excess microplate strips from the plate frame, return them to the foil pouch containing the desiccant pack, and reseal.
- Add 100 µL of Assay Diluent to each well.
- Add 50 µL of Standard, control, or sample to each well. Cover with a plate sealer, and incubate at room temperature for 2 hours on a horizontal orbital microplate shaker.
- Aspirate each well and wash, repeating the process 3 times for a total of 4 washes.
- Add 200 µL of Conjugate to each well. Cover with a new plate sealer, and incubate at room temperature for 2 hours on the shaker.
- Aspirate and wash 4 times.
- Add 200 µL Substrate Solution to each well. Incubate at room temperature for 30 minutes on the benchtop. PROTECT FROM LIGHT.
- Add 50 µL of Stop Solution to each well. Read at 450 nm within 30 minutes. Set wavelength correction to 540 nm or 570 nm.
100 µL Assay Diluent
50 µL Standard, Control, or Sample
200 µL Conjugate
200 µL Substrate Solution
50 µL Stop Solution
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