Human ErbB3/Her3 Quantikine ELISA Kit

R&D Systems | Catalog # DERB30

R&D Systems
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Key Product Details

Assay Length

4.5 hours

Sample Type & Volume Required Per Well

Cell Culture Supernates (50 µL), Cell Lysates (5 µg), Serum (10 µL), EDTA Plasma (10 µL), Heparin Plasma (10 µL), Saliva (10 µL), Urine (10 µL), Human Milk (10 µL)

Sensitivity

15.2 pg/mL

Assay Range

62.5-4000 pg/mL (Cell Culture Supernates, Cell Lysates, Serum, EDTA Plasma, Heparin Plasma, Saliva, Urine, Human Milk)
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Product Summary for Human ErbB3/Her3 Quantikine ELISA Kit

The Quantikine® Human ErbB3/Her3 Immunoassay is a 4.5 hour solid-phase ELISA designed to measure human ErbB3 in cell culture supernates, cell lysates, serum, plasma, saliva, urine and human milk. It contains NS0-expressed recombinant human ErbB3 and has been shown to accurately quantitate the recombinant factor. Results obtained using natural human ErbB3 showed linear curves that were parallel to the standard curves obtained using the Quantikine® kit standards. These results indicate that this kit can be used to determine relative mass values for natural human ErbB3.

Product Specifications

Measurement

Quantitative ELISA

Detection Method

Colorimetric - 450nm (TMB)

Conjugate

HRP

Species

Human

Specificity

Natural and recombinant human ErbB3.

Cross-reactivity

< 0.5% cross-reactivity observed with available related molecules. < 50% cross-species reactivity observed with species tested.

Interference

No significant interference observed with available related molecules.

精度

Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in twenty separate assays to assess inter-assay precision. Assays were performed by at least three technicians.

Cell Culture Supernates, Cell Lysates, EDTA Plasma, Heparin Plasma, Human Milk, Saliva, Serum, Urine

Intra-Assay Precision Inter-Assay Precision
Sample 1 2 3 1 2 3
n 20 20 20 20 20 20
Mean (pg/mL) 396 1176 2446 437 1317 2646
Standard Deviation 4.56 11.2 36.9 29.4 66.1 119
CV% 1.2 1.0 1.5 6.7 5.0 4.5

Recovery for Human ErbB3/Her3 Quantikine ELISA Kit

The recovery of human ErbB3 spiked to levels throughout the range of the assay in various matrices was evaluated.

Sample Type Average % Recovery Range %
Cell Culture Media (n=4) 97 92-100
Cell Lysis Buffer (n=2) 98 96-101
EDTA Plasma (n=4) 97 93-103
Heparin Plasma (n=4) 96 91-102
Human Milk (n=4) 96 91-103
Saliva (n=4) 100 94-106
Serum (n=4) 100 93-109
Urine (n=4) 97 94-102

Linearity

To assess the linearity of the assay, samples containing and/or spiked with high concentrations of human ErbB3 were diluted with calibrator diluent to produce samples with values within the dynamic range of the assay.

Human ErbB3/Her3 Linearity

Scientific Data Images for Human ErbB3/Her3 Quantikine ELISA Kit

Human ErbB3/Her3 ELISA Standard Curve

Human ErbB3/Her3 ELISA Standard Curve

Preparation and Storage

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: ErbB3/Her3

ErbB3, also called Her3 (human epidermal growth factor receptor 3) in humans, is a type I transmembrane glycoprotein that is a member of the ErbB family of tyrosine kinase receptors. ErbB3 binds neuregulins NRG1 and NRG2, but lacks a working kinase domain; heterodimerization with another ErbB receptor is required for signaling. ErbB3 is important in development of Schwann cells, neural crest cells, heart valves and the sympathetic nervous system. When expressed with ErbB2 in breast, skin, ovary and gastrointestinal tumors, ErbB3 is a potent mitogen.

Long Name

Receptor Tyrosine Protein Kinase ErbB3

Alternate Names

HER3

Entrez Gene IDs

2065 (Human); 13867 (Mouse)

Gene Symbol

ERBB3

Additional ErbB3/Her3 Products

Product Documents for Human ErbB3/Her3 Quantikine ELISA Kit

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

Product Specific Notices for Human ErbB3/Her3 Quantikine ELISA Kit

For research use only

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    Name: Anonymous
    Sample Tested: human floxp embryonal stem cell culture medium
    Verified Customer | Posted 11/22/2021
    Human ErbB3/Her3 Quantikine ELISA Kit DERB30

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