Human Fetuin B Antibody (212607)

R&D Systems | Catalog # MAB17251

R&D Systems

Key Product Details

Species Reactivity

Human

Applications

Western Blot, ELISA Capture (Matched Antibody Pair), Simple Western

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # 212607
View all Fetuin B Primary Antibodies »

Product Specifications

Immunogen

Mouse myeloma cell line NS0-derived recombinant human Fetuin B
Met19-Pro382
Accession # Q9UGM5

Specificity

Detects human Fetuin B in ELISAs. In sandwich immunoassays, no cross-reactivity or interference with recombinant human (rh) Fetuin A, rhCystatin A, B, C, rhMMP-2, 9, rhBMP-2, 4, 6, rhTGF-beta 1, or rhTGF-beta 2 is observed.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images for Human Fetuin B Antibody (212607)

Detection of Human Fetuin B antibody by Western Blot.

Detection of Human Fetuin B by Western Blot.

Western blot shows human serum. PVDF membrane was probed with 5 µg/mL of Mouse Anti-Human Fetuin B Monoclonal Antibody (Catalog # MAB17251) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for Fetuin B at approximately 55 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Detection of Human Fetuin B antibody by Simple WesternTM.

Detection of Human Fetuin B by Simple WesternTM.

Simple Western lane view shows lysates of human placenta tissue, loaded at 0.2 mg/mL. A specific band was detected for Fetuin B at approximately 62 kDa (as indicated) using 50 µg/mL of Mouse Anti-Human Fetuin B Monoclonal Antibody (Catalog # MAB17251). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system. Non-specific interaction with the 230 kDa Simple Western standard may be seen with this antibody.
Human Fetuin B ELISA Standard Curve

Human Fetuin B ELISA Standard Curve

Recombinant Human Fetuin B (Catalog # 1725-PI) was serially diluted and captured by Mouse Anti-Human Fetuin B Monoclonal Antibody (Catalog # MAB17251) coated on a Clear Polystyrene Microplate (Catalog # DY990). Mouse Anti-Human Fetuin B Biotinylated Monoclonal Antibody (Catalog # ) was incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)

Applications for Human Fetuin B Antibody (212607)

Application
Recommended Usage

Simple Western

50 µg/mL
Sample: Human placenta tissue

Western Blot

5 µg/mL
Sample: Human serum

Human Fetuin B Sandwich Immunoassay

ELISA Capture (Matched Antibody Pair)
Recommended Concentration: 2-8 µg/mL
Use in combination with these reagents:
  • Detection Reagent: Human Fetuin B Biotinylated Antibody (Catalog # BAM17251)
  • Standard: Recombinant Human Fetuin B Protein, CF (Catalog # 1725-PI)
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Reviewed Applications

Read 4 reviews rated 4.3 using MAB17251 in the following applications:

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Fetuin B

Fetuins are members of the cystatin superfamily of cysteine protease inhibitors (1‑3). Additional members of this superfamily are kininogen and histidine-rich glycoprotein. Fetuin A and B are two known members of the fetuin family. Hepatocytes are believed to be the principal cellular source, but other cell types also express it (4, 5). Fetuin A, also known as alpha 2-Heremans-Schmid glycoprotein, is an inhibitor of basic calcium phosphate precipitation and a negative acute-phase protein (6, 7). Normal circulating levels of Fetuin A in adults (300‑600 ug/mL) fall significantly (30‑50%) during injury and infection (7). Fetuin A and B display similarities and differences in their characteristics. Fetuin B exhibits reduction of calcification, while both mRNA levels were down‑regulated during the acute phase in inflammation-induced rats (4). However, they share only 20% amino acid sequence identity (2). The amounts of Fetuin B in human serum, unlike Fetuin A, vary with gender and are higher in females than in males (4).

References

  1. Oliver, E., et al. (1999) Genomics. 57:352.
  2. Oliver, E., et al. (2000) Biochem. J. 350:589.
  3. Kellemann, J. et al., 1989, J. Biol. Chem. 264:14121.
  4. Denecke, B. et al. (2003) Biochem. J. 376:135.
  5. Schäfer, C., et al. (2003) J. Clin. Invest. 112:357.
  6. Dziegielewska, K. M., et al. (1996) Histochem. Cell Biol. 106:319.
  7. Gangneux, C. et al. (2003) Nucleic Acids Res. 31:5957.

Alternate Names

FETUB

Entrez Gene IDs

26998 (Human)

Gene Symbol

FETUB

UniProt

Q9UGM5

Additional Fetuin B Products

Product Documents for Human Fetuin B Antibody (212607)

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human Fetuin B Antibody (212607)

For research use only

Related Research Areas

Cysteine Proteases and Regulators

Customer Reviews for Human Fetuin B Antibody (212607) (4)

4.3 out of 5
4 Customer Ratings
5 Stars
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4 Stars
75%
3 Stars
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1 Stars
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Showing  1 - 4 的 4 reviews Showing All
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  • Human Fetuin B Antibody
    Name: Anonymous
    Application: Western Blot
    Sample Tested: Serum
    Species: Human
    Verified Customer | Posted 11/12/2021
    Human Fetuin B Antibody (212607) MAB17251
  • Human Fetuin B Antibody
    Name: Anonymous
    Application: Microarrays
    Sample Tested: EDTA Plasma
    Species: Human
    Verified Customer | Posted 03/11/2019
    Human Fetuin B Antibody (212607) MAB17251
  • Human Fetuin B Antibody
    Name: Anonymous
    Application: ELISA
    Sample Tested: Serum and Plasma
    Species: Human
    Verified Customer | Posted 11/11/2018
  • Human Fetuin B Antibody
    Name: Anonymous
    Application: Microarray
    Sample Tested: EDTA Plasma
    Species: Human
    Verified Customer | Posted 11/09/2018

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