Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human, Mouse

Applications

Validated:

Western Blot, Neutralization

Cited:

Immunohistochemistry, Immunocytochemistry

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # 98707
View all FGFR2 Primary Antibodies »

Product Specifications

Immunogen

Mouse myeloma cell line NS0-derived recombinant human FGF R2 isoforms and S. frugiperda insect ovarian cell line Sf 21-derived recombinant human FGF R2 isoforms

Specificity

Reacts with multiple isoforms of human FGF R2 although it shows a marked preference for human FGF R2 (IIIb) isoforms in some assays as described below. In direct ELISAs, displays a three-fold preference for FGF R2 (IIIb) isoforms over (IIIc) isoforms. Shows approximately 50% cross-reactivity with recombinant mouse (rm) FGF R2 isoforms and no cross-reactivity with any isoforms of recombinant human (rh) FGF R1 or rhFGF R3. In Western blots, shows approximately 25% cross-reactivity with rhFGF R2 (IIIc) isoforms, 100% cross-reactivity with all isoforms of rmFGF R2 and no cross-reactivity with any isoforms of rhFGF RI, rhFGF R3, or rhFGF R4. Neutralizes the bioactivity of rhFGF R2 (IIIb) isoforms.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Endotoxin Level

<0.10 EU per 1 μg of the antibody by the LAL method.

Scientific Data Images for Human FGFR2 (IIIb) Antibody

FGF R2 alpha Inhibition of FGF acidic-dependent Cell Proliferation and Neutralization by Human FGF R2 Antibody.

FGF R2 alpha Inhibition of FGF acidic-dependent Cell Proliferation and Neutralization by Human FGF R2 Antibody.

Recombinant Human FGF R2a (IIIb) Fc Chimera (Catalog # 663-FR) inhibits Recombinant Human FGF acidic (Catalog # 232-FA) induced proliferation in the NR6R‑3T3 mouse fibroblast cell line in a dose-dependent manner (orange line). Inhibition of Recombinant Human FGF acidic (0.3 ng/mL) activity elicited by Recombinant Human FGF R2a (IIIb) Fc Chimera (6 ng/mL) is neutralized (green line) by increasing concentrations of Human FGF R2 (IIIb) Monoclonal Antibody (Catalog # MAB665). The ND50 is typically 2-8 µg/mL in the presence of heparin (10 µg/mL).

Applications for Human FGFR2 (IIIb) Antibody

Application
Recommended Usage

Western Blot

1 µg/mL
Sample: Recombinant Human FGF R2 beta (IIIb) Fc Chimera (Catalog # 665-FR)

Neutralization

Measured by its ability to neutralize FGF R2 alpha -mediated inhibition of proliferation in the NR6R‑3T3 mouse fibroblast cell line. The Neutralization Dose (ND50) is typically 2-8 µg/mL in the presence of 6 ng/mL Recombinant Human FGF R2 alpha (IIIb) Fc Chimera, 0.3 ng/mL Recombinant Human FGF acidic, and 10 µg/mL heparin.

Formulation, Preparation, and Storage

Purification

Protein A or G purified from ascites

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

=
÷

Background: FGFR2

FGF R2 is a transmembrane tyrosine kinase that functions as a receptor for several FGF family proteins. The extracellular domain includes three ( alpha isoforms) or two ( beta isoforms) Ig-like domains. Alternative exon usage results in IIIb or IIIc isoforms that differ in their membrane proximal Ig-like domain.

Long Name

Fibroblast Growth Factor Receptor 2

Alternate Names

CD332, FGF R2

Entrez Gene IDs

2263 (Human); 14183 (Mouse)

Gene Symbol

FGFR2

Additional FGFR2 Products

Product Documents for Human FGFR2 (IIIb) Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

Download SDS

Product Specific Notices for Human FGFR2 (IIIb) Antibody

For research use only

Citations for Human FGFR2 (IIIb) Antibody

Customer Reviews for Human FGFR2 (IIIb) Antibody

There are currently no reviews for this product. Be the first to review Human FGFR2 (IIIb) Antibody and earn rewards!

Have you used Human FGFR2 (IIIb) Antibody?

Submit a review and receive an Amazon gift card!

$25/€18/£15/$25CAN/¥2500 Yen for a review with an image

$10/€7/£6/$10CAN/¥1110 Yen for a review without an image

Submit a review
Amazon Gift Card

FAQs

No product specific FAQs exist for this product.

View all FAQs for Antibodies