Human Glycine N-methyltransferase/GNMT Alexa Fluor® 350-conjugated Antibody

Catalog #: FAB6526U Datasheet / COA / SDS

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FAB6526U-100UG

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All Glycine N-Methyltransferase/GNMT Antibodies

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Summary Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human Glycine N-methyltransferase/GNMT Alexa Fluor® 350-conjugated Antibody Summary

Species Reactivity

Human

Specificity

Detects human Glycine N-methyltransferase/GNMT in direct ELISAs.

Source

Monoclonal Mouse IgG1 Clone # 691305

Purification

Protein A or G purified

Immunogen

E. coli-derived recombinant human Glycine N-methyltransferase/GNMT
Met1-Asp295
Accession # Q14749

Formulation

Supplied 0.2mg/ml in 1X PBS with RDF1 and 0.09% Sodium Azide

Label

Alexa Fluor 350 (Excitation= 346 nm, Emission= 442 nm)

Applications

Recommended Concentration

Sample

Western Blot

Optimal dilution of this antibody should be experimentally determined.

Immunoprecipitation

Optimal dilution of this antibody should be experimentally determined.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied

Background: Glycine N-Methyltransferase/GNMT

GNMT (glycine N-methyltransferase) is a cytoplasmic homotetramer of 33 kDa units that converts S-adenosylmethionine to N-methylglycine (sarcosine), also producing S-adenosylhomocysteine. It is abundant in liver, but has also been found in the pancreas and prostate, and is downregulated in hepatocellular carcinoma. GNMT adjusts liver methyl group synthesis for dietary methionine, influencing DNA methylation. The 5-methyltetrahydrofolate form of folic acid binds and inhibits GNMT activity. The 295 amino acid (aa) human GNMT shares 92% aa identity with mouse and rat GNMT.

Entrez Gene IDs

27232 (Human); 14711 (Mouse); 25134 (Rat)

Alternate Names

EC 2.1.1.20; Glycine NMethyltransferase; Glycine N-Methyltransferase; GNMT