Human Glycine N-Methyltransferase/GNMT Antibody (691305)

R&D Systems | Catalog # MAB6526

R&D Systems

Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Validated:

Western Blot, Immunoprecipitation

Cited:

Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # 691305
View all Glycine N-Methyltransferase/GNMT Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant human Glycine N-methyltransferase/GNMT
Met1-Asp295
Accession # Q14749

Specificity

Detects human Glycine N-methyltransferase/GNMT in direct ELISAs.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images for Human Glycine N-Methyltransferase/GNMT Antibody (691305)

Detection of Mouse Glycine N-methyltransferase/GNMT antibody by Western Blot.

Detection of Mouse Glycine N-methyltransferase/GNMT by Western Blot.

Western blot shows lysates of mouse liver tissue. PVDF Membrane was probed with 1 µg/mL of Human Glycine N-methyltransferase/GNMT Monoclonal Antibody (Catalog # MAB6526) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for Glycine N-methyltransferase/GNMT at approximately 37 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Applications for Human Glycine N-Methyltransferase/GNMT Antibody (691305)

Application
Recommended Usage

Immunoprecipitation

25 µg/mL
Sample: Cell lysates spiked with recombinant human Glycine N-methyltransferase/GNMT, see our available Western blot detection antibodies

Western Blot

1 µg/mL
Sample: Mouse liver tissue

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Sterile PBS to a final concentration of 0.5 mg/mL. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

=
÷

Background: Glycine N-Methyltransferase/GNMT

GNMT (glycine N-methyltransferase) is a cytoplasmic homotetramer of 33 kDa units that converts S-adenosylmethionine to N-methylglycine (sarcosine), also producing S-adenosylhomocysteine. It is abundant in liver, but has also been found in the pancreas and prostate, and is downregulated in hepatocellular carcinoma. GNMT adjusts liver methyl group synthesis for dietary methionine, influencing DNA methylation. The 5-methyltetrahydrofolate form of folic acid binds and inhibits GNMT activity. The 295 amino acid (aa) human GNMT shares 92% aa identity with mouse and rat GNMT.

Alternate Names

Glycine NMethyltransferase, GNMT

Entrez Gene IDs

27232 (Human); 14711 (Mouse); 25134 (Rat)

Gene Symbol

GNMT

UniProt

Q14749 (Human)

Additional Glycine N-Methyltransferase/GNMT Products

Product Documents for Human Glycine N-Methyltransferase/GNMT Antibody (691305)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

Download SDS

Product Specific Notices for Human Glycine N-Methyltransferase/GNMT Antibody (691305)

For research use only

Citations for Human Glycine N-Methyltransferase/GNMT Antibody (691305)

Customer Reviews for Human Glycine N-Methyltransferase/GNMT Antibody (691305)

There are currently no reviews for this product. Be the first to review Human Glycine N-Methyltransferase/GNMT Antibody (691305) and earn rewards!

Have you used Human Glycine N-Methyltransferase/GNMT Antibody (691305)?

Submit a review and receive an Amazon gift card!

$25/€18/£15/$25CAN/¥2500 for a review with an image

$10/€7/£6/$10CAN/¥1110 for a review without an image

Submit a review
Amazon Gift Card

FAQs

No product specific FAQs exist for this product.

View all FAQs for Antibodies