HGF Activator Inhibitor type II (HAI-2), also known as placental bikunin, is a Kunitz-type protease inhibitor. It is a type I membrane protein containing 2 Kunitz inhibitor domains in its extracellular region. In mouse, at least three isoforms of HAI-2 exist as a result of alternative splicing. Isoforms 2 and 3, also known as HAI-2B and HAI-2C, respectively, are both missing the first amino-terminal Kunitz inhibitor domain.
Key Product Details
Species Reactivity
Validated:
Human
Cited:
Human
Applications
Validated:
Western Blot, ELISA Capture (Matched Antibody Pair)
Cited:
Immunohistochemistry-Paraffin, Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 Clone # 170018
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Product Specifications
Immunogen
Mouse myeloma cell line NS0-derived recombinant human HAI-2
aa 28-197
Accession # O43291
aa 28-197
Accession # O43291
Specificity
Detects recombinant human (rh)HAI-2 in direct ELISAs and Western blots. In these formats, does not cross-react with rhHAI-1 or rmHAI-2B.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1
Applications for Human HAI‑2 Ectodomain Antibody
Application
Recommended Usage
Western Blot
1 µg/mL
Sample: Recombinant Human HAI‑2 (Catalog # 1106-PI)
Sample: Recombinant Human HAI‑2 (Catalog # 1106-PI)
Human HAI-2 Sandwich Immunoassay
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Reviewed Applications
Read 3 reviews rated 3.7 using MAB1106 in the following applications:
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
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Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Calculators
Background: HAI-2
Long Name
HGF Activator Inhibitor Type 2
Alternate Names
HAI2
Gene Symbol
SPINT2
UniProt
Additional HAI-2 Products
Product Documents for Human HAI‑2 Ectodomain Antibody
Product Specific Notices for Human HAI‑2 Ectodomain Antibody
For research use only
Related Research Areas
Citations for Human HAI‑2 Ectodomain Antibody
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Customer Reviews for Human HAI‑2 Ectodomain Antibody (3)
3.7 out of 5
3 Customer Ratings
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Application: MicroarraysSample Tested: EDTA PlasmaSpecies: HumanVerified Customer | Posted 01/14/2021
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- Cellular Response to Hypoxia Protocols
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- Troubleshooting Guide: Western Blot Figures
- Western Blot Conditions
- Western Blot Protocol
- Western Blot Protocol for Cell Lysates
- Western Blot Troubleshooting
- Western Blot Troubleshooting Guide
- View all Protocols, Troubleshooting, Illustrated assays and Webinars