Key Product Details

Species Reactivity

Human

Applications

Western Blot, Simple Western

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # 906705
View all HHEX Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant human HHEX 
Thr111-Gly270
Accession # Q03014

Specificity

Detects human HHEX in direct ELISAs.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images for Human HHEX Antibody

Detection of Human HHEX antibody by Western Blot.

Detection of Human HHEX by Western Blot.

Western blot shows lysates of K562 human chronic myelogenous leukemia cell line and HepG2 human hepatocellular carcinoma cell line. PVDF membrane was probed with 2 µg/mL of Mouse Anti-Human HHEX Monoclonal Antibody (Catalog # MAB8377) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for HHEX at approximately 37 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Detection of Human HHEX antibody by Simple WesternTM.

Detection of Human HHEX by Simple WesternTM.

Simple Western lane view shows lysates of K562 human chronic myelogenous leukemia cell line, loaded at 0.5 mg/mL. A specific band was detected for HHEX at approximately 47 kDa (as indicated) using 20 µg/mL of Mouse Anti-Human HHEX Monoclonal Antibody (Catalog # MAB8377). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.

Applications for Human HHEX Antibody

Application
Recommended Usage

Simple Western

20 µg/mL
Sample: K562 human chronic myelogenous leukemia cell line

Western Blot

2 µg/mL
Sample: K562 human chronic myelogenous leukemia cell line and HepG2 human hepatocellular carcinoma cell line

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

=
÷

Background: HHEX

Hematopoietically-expressed homeobox protein (HHEX), also known as HEX, PRH and PRHX, is a 35-40 kDa member of the Homeobox family of transcription factors.  Family members are distinguished by an evolutionarily conserved DNA-binding homeodomain of 60 amino acids (aa), which for HHEX spans aa 137-196.  Human HHEX was initially isolated from hematopoietic tissue, and is present in several hematopoietic progenitors, where its expression is down-regulated during terminal cell differentiation.  HHEX is also expressed in the anterior visceral endoderm during early mouse development, and in some adult tissues of endodermal origin, including liver, lung and thyroid.  HHEX knockout in mice is embryonic lethal, with impaired forebrain, liver and thyroid development.  Human HHEX is 270 aa in length, and over aa 111-270, shares 93% and 95% identity with mouse and rat HHEX, respectively.

Long Name

Hematopoietically Expressed Homeobox

Alternate Names

Hex, HMPH, HOX11L-PEN, PRH, PRHX

Entrez Gene IDs

3087 (Human); 15242 (Mouse); 79237 (Rat)

Gene Symbol

HHEX

UniProt

Q03014

Additional HHEX Products

Product Documents for Human HHEX Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

Download SDS

Product Specific Notices for Human HHEX Antibody

For research use only

Customer Reviews for Human HHEX Antibody

There are currently no reviews for this product. Be the first to review Human HHEX Antibody and earn rewards!

Have you used Human HHEX Antibody?

Submit a review and receive an Amazon gift card!

$25/€18/£15/$25CAN/¥2500 Yen for a review with an image

$10/€7/£6/$10CAN/¥1110 Yen for a review without an image

Submit a review
Amazon Gift Card

FAQs

No product specific FAQs exist for this product.

View all FAQs for Antibodies