Human IkB-epsilon Antibody (418515)

Name: Human IkB-epsilon Antibody (418515)
Brand: R&D Systems
SKU: MAB4300
Price: 399 USD
Availability: InStock

R&D Systems | Catalog # MAB4300

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Product Specifications
Scientific Data
Applications
Preparation & Storage
Calculators
Background
Product Documents
Specific Notices
Research Areas

Key Product Details

Species Reactivity

Human

Applications

Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG_2B Clone # 418515

View all IkB-epsilon Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant human IkB-epsilon
Ser64-Asp423
Accession # Q96F31

Specificity

Detects endogenous human and mouse IkB-epsilon in Western blots.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG_2B

Scientific Data Images for Human IkB-epsilon Antibody (418515)

Detection of Human IkB-epsilon by Western Blot.

Western blot shows lysates of DA3 mouse myeloma cell line, PC-3 human prostate cancer cell line, Raji human Burkitt's lymphoma cell line, LNCaP human prostate cancer cell line, and MCF-7 human breast cancer cell line. PVDF membrane was probed with 1 µg/mL of Human IkB-e Monoclonal Antibody (Catalog # MAB4300) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for IkB-e at approximately 45 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 4.

Applications for Human IkB-epsilon Antibody (418515)

Application

Recommended Usage

Western Blot

1 µg/mL
Sample: DA3 mouse myeloma cell line, Raji human Burkitt's lymphoma cell line, MCF-7 human breast cancer cell line, PC-3 human prostate cancer cell line, and LNCaP human prostate cancer cell line

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

Volume (to add to vial)

Mass (in vial)

Desired Reconstitution Concentration

Background: IkB-epsilon

NF kappa B inhibitor epsilon (IkB epsilon ) inhibits the NF kappa B complex by binding and sequestering it in the cytoplasm. Upon stimulation, IkB epsilon is phosphorylated on serine residues marking it for degradation by the ubiquitin pathway. Following IkB epsilon degradation, the NF kappa B complex is able to translocate to the nucleus and activate transcription.