Human IL-11 R alpha Antibody (1020854)

R&D Systems | Catalog # MAB19771

R&D Systems

Key Product Details

Species Reactivity

Human

Applications

Western Blot, Simple Western

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2B Clone # 1020854
View all IL-11 R alpha Primary Antibodies »

Product Specifications

Immunogen

Chinese Hamster Ovary cell line, CHO-derived human IL-11 R alpha
Ser24-Val363
Accession # Q14626

Specificity

Detects human IL-11 R alpha in direct ELISAs.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2B

Scientific Data Images for Human IL-11 R alpha Antibody (1020854)

Detection of Human, Mouse, and Rat IL-11 Ra antibody by Western Blot.

Detection of Human, Mouse, and Rat IL‑11 R alpha by Western Blot.

Western blot shows lysates of human skeletal muscle tissue, mouse skeletal muscle tissue, and rat skeletal muslce tissue. PVDF membrane was probed with 2 µg/mL of Mouse Anti-Human IL-11 Ra Monoclonal Antibody (Catalog # MAB19771) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for IL-11 Ra at approximately 45-49 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Detection of Human IL‑11 R alpha by Simple WesternTM.

Simple Western lane view shows lysates of human skeletal muscle, loaded at 0.2 mg/mL. A specific band was detected for IL‑11 R alpha at approximately 49 kDa (as indicated) using 50 µg/mL of Mouse Anti-Human IL‑11 R alpha Monoclonal Antibody (Catalog # MAB19771). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.

Applications for Human IL-11 R alpha Antibody (1020854)

Application
Recommended Usage

Simple Western

50 µg/mL
Sample: Human skeletal muscle

Western Blot

2 µg/mL
Sample: Human skeletal muscle tissue, Mouse skeletal muscle tissue, and Rat skeletal muslce tissue

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: IL-11 R alpha

Interleukin-11 receptor alpha (IL-11 R alpha ) is a 49 kDa type I transmembrane member of the gp130 subfamily of the hematopoietic cytokine receptor family (1-3). Mature human IL-11 R alpha consists of a 347 amino acid (aa) extracellular domain (ECD) that contains a C2 type Ig-like domain, two fibronectin type III domains, two potential glycosylation sites, and a WSxWS motif, followed by a 21 aa transmembrane region and a 31 aa cytoplasmic domain (4). Within the ECD, human IL-11 R alpha shares 84%, 82%, 90%, and 86% aa sequence identity with mouse, rat, equine, and bovine IL-11 R alpha, respectively. Alternative splicing generates an additional isoform that lacks the cytoplasmic domain (4). Upon low affinity binding to IL-11, IL-11 R alpha associates with gp130 to form a high affinity receptor complex (1, 3). gp130 also functions as a subunit in the receptors for Cardiotrophin-1, CNTF, IL-6, IL-27, IL-31, LIF, and Oncostatin M (5). IL-11 R alpha is widely expressed in adults, embryos, and embryonic stem cells (4, 6, 7). Deletion of IL-11 R alpha in female mice causes faulty decidualization, lack of decidual NK cells, and infertility (8-10). IL-11 is anti-apoptotic for oligodendrocytes, and lack of IL-11 R alpha increases the severity of experimental autoimmune encephalitis (11, 12). IL-11 R alpha is also anti-apoptotic for colonic epithelia, and increased IL-11 signaling may be a factor in inflammation-associated gastrointestinal cancer development (3, 13). IL-11 R alpha enhances osteoclast differentiation and bone remodeling but inhibits adipocyte differentiation (1, 2). Recombinant soluble IL-11 R alpha confers IL-11 responsiveness to cells expressing gp130, while in cells expressing transmembrane IL-11 R alpha and gp130, soluble IL-11 R alpha acts as an IL-11 antagonist (14-16).

References

  1. Sims, N.A. and N.C. Walsh (2010) BMB Reports 43:513.
  2. White, U.A. and J.M. Stephens (2011) Curr. Pharm. Des. 17:340.
  3. Kiessling, S. et al. (2004) J. Biol. Chem. 279:10304.
  4. Cherel, M. et al. (1995) Blood 86:2534.
  5. Garbers, C. et al. (2012) Cytokine Growth Factor Rev. 23:85.
  6. Hilton, D.J. et al. (1994) EMBO J. 13:4765.
  7. Davidson, A.J. et al. (1997) Stem Cells 15:119.
  8. Paiva, P. et al. (2009) Cytokine Growth Factor Rev. 20:319.
  9. Robb, L. et al. (1998) Nat. Med. 4:303.
  10. Ain, R. et al. (2004) Dev. Dyn. 231:700.
  11. Gurfein, B.T. et al. (2009) J. Immunol. 183:4229.
  12. Zhang, J. et al. (2011) J. Immunol. 187:1129.
  13. Putoczki, T. and M. Ernst (2010) J. Leukoc. Biol. 88:1109.
  14. Curtis, D.J. et al. (1997) Blood 90:4403.
  15. Baumann, H. et al. (1996) J. Immunol. 157:284.
  16. Karow, J. et al. (1996) Biochem. J. 318:489.

Long Name

Interleukin 11 Receptor alpha

Alternate Names

IL-11Ra, IL11R alpha, IL11RA

Entrez Gene IDs

3590 (Human); 16157 (Mouse)

Gene Symbol

IL11RA

UniProt

Q14626

Additional IL-11 R alpha Products

Product Documents for Human IL-11 R alpha Antibody (1020854)

Certificate of Analysis

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Product Specific Notices for Human IL-11 R alpha Antibody (1020854)

For research use only

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