Human IL-17/IL-17A (Research Grade Secukinumab Biosimilar) Antibody (Hu71)

Name: Human IL-17/IL-17A (Research Grade Secukinumab Biosimilar) Antibody (Hu71)
Brand: R&D Systems
SKU: MAB9492
Price: 169 USD
Availability: InStock

R&D Systems | Catalog # MAB9492

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Product Documents

Recombinant Monoclonal Antibody For Research Use Only

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Product Specifications
Scientific Data
Applications
Preparation & Storage
Calculators
Background
Product Documents
Specific Notices
Research Areas

Key Product Details

Species Reactivity

Human

Applications

ELISA

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Human IgG₁ Clone # Hu71

View all IL-17/IL-17A Primary Antibodies »

Product Specifications

Immunogen

Human IL‑17/IL‑17A

Clonality

Monoclonal

Host

Human

Isotype

IgG₁

Scientific Data Images

Human IL‑17/IL‑17A ELISA Standard Curve

Direct ELISA binding curve demonstrating the recognition of Human Anti-Human IL‑17/IL‑17A (Research Grade Secukinumab Biosimilar) Monoclonal Antibody (Catalog # MAB9492) to IL‑17/IL‑17A. The target protein was coated onto the microplate well surface, followed by binding of the antibody. A goat anti-human HRP conjugate was used for detection.

Applications

Application

Recommended Usage

ELISA

This antibody functions as an ELISA detection antibody for the specific antigen in direct ELISA. Colorimetric detection is performed after addition of a suitable substrate.

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

Volume (to add to vial)

Mass (in vial)

Desired Reconstitution Concentration

Background: IL-17/IL-17A

Secukinumab is a humanized monoclonal IgG1/k antibody against proinflammatory cytokine IL-17A, and is approved for the treatment of plaque psoriasis, as well as enthesitis-related arthritis, and ankylosing spondylitis. Secukinumab binds to IL-17A with high specificity preventing the interaction with the IL-17 receptor and subsequent activation of the pathway leading to inflammation seen in these disease states. Data has shown that in the presence of secukinumab results in enhanced levels of free unbound IL-17A in the serum indicating inhibition of binding to IL-17A receptor resulting in reduced inflammation.