Human IQGAP1 Alexa Fluor® 488-conjugated Antibody

Catalog #: AF5069G Datasheet / COA / SDS

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AF5069G-100UG

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Human IQGAP1 Alexa Fluor® 488-conjugated Antibody Summary

Species Reactivity

Human

Specificity

Detects endogenous human IQGAP1 in Western blots.

Source

Polyclonal Sheep IgG

Purification

Antigen Affinity-purified

Immunogen

E. coli-derived recombinant human IQGAP1
Lys505-Ser717
Accession # P46940

Formulation

Supplied 0.2mg/ml in 1X PBS with RDF1 and 0.09% Sodium Azide

Label

Alexa Fluor 488 (Excitation= 488 nm, Emission= 515-545 nm)

Applications

Recommended Concentration

Sample

Western Blot

Optimal dilution of this antibody should be experimentally determined.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied

Background: IQGAP1

IQGAP1 (IQ motif with Ras GAP-related domain 1) is a 195 kDa member of the IQGAP family of proteins. It serves as an effector protein for Rho family members, and regulates actin polymerization. Human IQGAP1 is 1657 amino acids (aa) in length. It contains one actin-binding CH domain (aa 45‑159), a Pro-binding WWP region (aa 685‑711), four consecutive calmodulin-binding IQ motifs (aa 745‑864), and a noncatalytic Ras-GAP domain (aa 1004‑1237). Amino acids 763‑863 mediate homodimerization. There are at least three IQGAP1 splice variants. One shows a two Lys substitution for aa 270‑1657, a second shows a ten Lys substitution for aa 299‑1657, and a third shows a three Lys substitution for aa 941‑1657.

Long Name

IQ Motif Containing GTPase Activating Protein 1

Entrez Gene IDs

8826 (Human); 544963 (Mouse)

Alternate Names

HUMORFA01; IQ motif containing GTPase activating protein 1; IQGAP1; KIAA0051ras GTPase-activating-like protein IQGAP1; p195; p195RasGAP-like with IQ motifs; SAR1