Human IRF1 Alexa Fluor® 532-conjugated Antibody

Catalog #: FAB4830X Datasheet / COA / SDS

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FAB4830X-100UG

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Human IRF1 Alexa Fluor® 532-conjugated Antibody Summary

Species Reactivity

Human

Specificity

Detects human IRF1 in direct ELISAs and Western blots. In direct ELISAs and Western blots, no cross-reactivity with recombinant human (rh) IRF2 (aa 151-349), rhIRF3 (aa 206-427), rhIRF4 (aa 130-451), rhIRF5 (aa 214-395), rhIRF6 (aa 176-366), rhIRF7 (

Source

Monoclonal Mouse IgG2b Clone # 686703

Purification

IgM-specific Affinity-purified

Immunogen

E. coli-derived recombinant human IRF1
Thr147-Pro325
Accession # P10914

Formulation

Supplied 0.2mg/ml in 1X PBS with RDF1 and 0.09% Sodium Azide

Label

Alexa Fluor 532 (Excitation= 534 nm, Emission= 553 nm)

Applications

Recommended Concentration

Sample

Western Blot

Optimal dilution of this antibody should be experimentally determined.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

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Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied

Background: IRF1

IRF1 (interferon regulatory factor 1) is a 45 kDa member of the IRF family of proteins. It is induced by TLR9 and INF-gamma stimulation, and positively regulates the expression of IFN-beta, iNOS and 1L-12 p35. Human IRF1 is 325 amino acids (aa) in length. It contains an N-terminal DNA-binding basic region (aa 1-140) and an acidic C-terminus (aa 141-325). Lys275 and 299 can be SUMOylated, which blocks activity, or ubiquinated at Lys299, which initiates degradation. There is a splice variant that shows an 11 aa substitution for the C-terminal 86 amino acids. Over aa 171-325, human IRF1 shares 83% aa identity with both canine and porcine IRF1.

Long Name

Interferon Regulatory Factor 1

Entrez Gene IDs

3659 (Human); 16362 (Mouse); 24508 (Rat)

Alternate Names

interferon regulatory factor 1; IRF1; MAR; MARIRF-1