Human IRF1 Alexa Fluor® 594-conjugated Antibody

Catalog #: AF4830T Datasheet / COA / SDS

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AF4830T-100UG

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Human IRF1 Alexa Fluor® 594-conjugated Antibody Summary

Species Reactivity

Human

Specificity

Detects human IRF1 in direct ELISAs and Western blots. In direct ELISAs, approximately 60% cross-reactivity with recombinant mouse IRF1 is observed.

Source

Polyclonal Goat IgG

Purification

Antigen Affinity-purified

Immunogen

E. coli-derived recombinant human IRF1
Thr147-Pro325
Accession # P10914

Formulation

Supplied 0.2mg/ml in 1X PBS with RDF1 and 0.09% Sodium Azide

Label

Alexa Fluor 594 (Excitation= 590 nm, Emission= 617 nm)

Applications

Recommended Concentration

Sample

Western Blot

Optimal dilution of this antibody should be experimentally determined.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied

Background: IRF1

IRF1 (interferon regulatory factor 1) is a 45 kDa member of the IRF family of proteins. It is induced by TLR9 and INF-gamma stimulation, and positively regulates the expression of IFN-beta, iNOS and 1L-12p35. Human IRF1 is 325 amino acids (aa) in length. It contains an N-terminal DNA-binding basic region (aa 1‑140) and an acidic C-terminus (aa 147‑325). Lys275 and 299 can be SUMOylated, which blocks activity, or ubiquinated at Lys299, which initiates degradation. There is a splice variant that shows an 11 aa substitution for the C-terminal 86 amino acids. Over aa 171‑325, human IRF1 shares 83% aa identity with both canine and porcine IRF1.

Long Name

Interferon Regulatory Factor 1

Entrez Gene IDs

3659 (Human); 16362 (Mouse); 24508 (Rat)

Alternate Names

interferon regulatory factor 1; IRF1; MAR; MARIRF-1