Human IRF4 Antibody (503210)

R&D Systems | Catalog # MAB55251

R&D Systems

Key Product Details

Species Reactivity

Human

Applications

Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2B Clone # 503210
View all IRF4 Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant human IRF4
Glu130-Glu451
Accession # Q15306

Specificity

Detects human IRF4 in direct ELISAs.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2B

Scientific Data Images for Human IRF4 Antibody (503210)

Detection of Human IRF4 by Western Blot.

Western blot shows lysates of Ramos human Burkitt's lymphoma cell line, HDLM‑2 human Hodgkin’s lymphoma cell line, U937 human histiocytic lymphoma cell line (negative control), and HUVEC human umbilical vein endothelial cells (negative control). PVDF membrane was probed with 2 µg/mL of Mouse Anti-Human IRF4 Monoclonal Antibody (Catalog # MAB55251) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (HAF018). A specific band was detected for IRF4 at approximately 51 kDa (as indicated). This experiment was conducted under reducing conditions and using Western Blot Buffer Group 1.

Applications for Human IRF4 Antibody (503210)

Application
Recommended Usage

Western Blot

2 µg/mL
Sample: Ramos human Burkitt's lymphoma cell line and HDLM‑2 human Hodgkin's lymphoma cell line

Reviewed Applications

Read 1 review rated 5 using MAB55251 in the following applications:

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: IRF4

Interferon Regulatory Factor 4 (IRF4), also known as MUM1 and LSIRF, is a 51 kDa lymphocyte-restricted transcription factor. It is required for immunoglobulin class switching and terminal differentiation of B cells. IRF4 is overexpressed in multiple myeloma and cooperates with Myc in an autoregulatory loop. In T cells, IRF4 is required for the production of IL-4. IRF4 contains an N-terminal DNA binding domain that is homologous to that in other IRF proteins. Within the C-terminal domain (aa 130‑451), human IRF4 shares 90% aa sequence identity with mouse and rat IRF4. Alternate splicing may generate isoforms with N-terminal, C-terminal, or internal deletions.

Long Name

Interferon Regulatory Factor 4

Alternate Names

LSIRF, MUM1, NF-EM5

Entrez Gene IDs

3662 (Human); 16364 (Mouse); 291100 (Rat)

Gene Symbol

IRF4

UniProt

Q15306

Additional IRF4 Products

Product Documents for Human IRF4 Antibody (503210)

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human IRF4 Antibody (503210)

For research use only

Customer Reviews for Human IRF4 Antibody (503210) (1)

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  • Human IRF4 Antibody
    Name: Anonymous
    Application: Western Blot
    Sample Tested: HDLM‑2 human Hodgkin’s lymphoma cell line
    Species: Human
    Verified Customer | Posted 02/05/2022
    Human IRF4 Antibody (503210) MAB55251

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