Human Laforin/EPM2A Antibody (523435)

R&D Systems | Catalog # MAB5714

R&D Systems

Key Product Details

Species Reactivity

Human

Applications

Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2B Clone # 523435
View all Laforin/EPM2A Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant human Laforin/EPM2A
Met1-Leu331
Accession # AAH70047

Specificity

Detects human Laforin/EPM2A in Western blots.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2B

Scientific Data Images for Human Laforin/EPM2A Antibody (523435)

Detection of Human Laforin/EPM2A antibody by Western Blot.

Detection of Human Laforin/EPM2A by Western Blot.

Western blot shows lysates of human heart and brain tissue, and HeLa human cervical epithelial carcinoma cell line. PVDF membrane was probed with 1 µg/mL Mouse Anti-Human Laforin/EPM2A Monoclonal Antibody (Catalog # MAB5714) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). For additional reference, recombinant human Laforin (3 ng) was included. A specific band for Laforin/EPM2A was detected at approximately 38 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 3.

Applications for Human Laforin/EPM2A Antibody (523435)

Application
Recommended Usage

Western Blot

1 µg/mL
Sample: Human heart and brain tissue, and HeLa human cervical epithelial carcinoma cell line

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Laforin/EPM2A

Laforin, also known as Lafora PTPase and EPM2A, is a 38 kDa member of the protein tyrosine phosphatase family. Human Laforin is 331 aa in length and contains one carbohydrate binding type-20 (CBM20) domain (aa 1-124) and one tyrosine-protein phosphatase domain (aa 243-311). Multiple splicing variants produce four isoforms of human Laforin, which is most highly expressed in heart, skeletal muscle, kidney, pancreas and brain. It functions as a dual specificity protein phosphatase and may be involved in the control of glycogen metabolism. Mutations in Laforin cause progressive myoclonic epilepsy type 2, also known as Lafora disease.

Alternate Names

EPM2A, LAFPTPase, LDE, MELF

Entrez Gene IDs

7957 (Human); 13853 (Mouse); 680683 (Rat)

Gene Symbol

EPM2A

UniProt

AAH70047

Additional Laforin/EPM2A Products

Product Documents for Human Laforin/EPM2A Antibody (523435)

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human Laforin/EPM2A Antibody (523435)

For research use only

Related Research Areas

Phosphatases and Regulators

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