Human LILRA2/CD85h/ILT1 Antibody (600002)

R&D Systems | Catalog # MAB63641

R&D Systems

Key Product Details

Species Reactivity

Human

Applications

Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2B Clone # 600002
View all LILRA2/CD85h/ILT1 Primary Antibodies »

Product Specifications

Immunogen

Mouse myeloma cell line NS0-derived recombinant human LILRA2/CD85h/ILT1
Pro17-Ser437
Accession # Q8N149

Specificity

Detects human LILRA-2 in direct ELISAs. In direct ELISAs, no cross-reactivity with recombinant human (rh) LILRA-1, rhLILRA-3, rhLILRA-4, rhLILRA-5, rhLILRA-6, rhILT-2, rhILT-4, or rhILT-5 is observed.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2B

Scientific Data Images for Human LILRA2/CD85h/ILT1 Antibody (600002)

Detection of Human LILRA2/CD85h/ILT1 antibody by Western Blot.

Detection of Human LILRA2/CD85h/ILT1 by Western Blot.

Western blot shows lysates of human peripheral blood mononuclear cells (PBMC). PVDF membrane was probed with 2 µg/mL of Mouse Anti-Human LILRA2/CD85h/ILT1 Monoclonal Antibody (Catalog # MAB63641) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for LILRA2/CD85h/ILT1 at approximately 69 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Applications for Human LILRA2/CD85h/ILT1 Antibody (600002)

Application
Recommended Usage

Western Blot

2 µg/mL
Sample: human peripheral blood mononuclear cells (PBMC)

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: LILRA2/CD85h/ILT1

Immune-like transcript 1 (ILT1), also known as leukocyte immunoglobulin-like receptor 7 (LIR-7), leukocyte immunoglobulin-like receptor subfamily A member 2 (LILRA2), and CD85H, is a 51 kDa (unglycosylated) type I transmembrane glycoprotein and member of the leukocyte immunoglobulin-like receptor family. Human ILT1 is synthesized as a 483 amino acid (aa) precursor that contains a 23 aa signal sequence, a 426 aa extracellular domain (ECD), a 21 aa transmembrane segment, and a 13 aa cytoplasmic region. The ECD contains four Ig-like C2-type domains and seven potential sites for N-linked glycosylation. A splice variant produces a second isoform that has a one aa substitution for aa 419-436 in the longer form. There are no murine orthologs for human ILT1. ILT1 may act as a receptor for class I MHC antigens.

Long Name

Leukocyte Immunoglobulin-like Receptor, Subfamily A (with TM domain), Member 2

Alternate Names

CD85h, ILT1, LIR7

Entrez Gene IDs

11027 (Human)

Gene Symbol

LILRA2

UniProt

Q8N149 (Human)

Additional LILRA2/CD85h/ILT1 Products

Product Documents for Human LILRA2/CD85h/ILT1 Antibody (600002)

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human LILRA2/CD85h/ILT1 Antibody (600002)

For research use only

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