Human LNK/SH2B3 Alexa Fluor® 700-conjugated Antibody

Catalog #: AF5888N Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
AF5888N-100UG

Save 15% on Select RUO Reagents. See Details.

All LNK/SH2B3 Antibodies

Product Details

Supplemental Products

Reviews

Summary Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human LNK/SH2B3 Alexa Fluor® 700-conjugated Antibody Summary

Species Reactivity

Human

Specificity

Detects human LNK/SH2B3 in Western blots.

Source

Polyclonal Sheep IgG

Purification

Antigen Affinity-purified

Immunogen

E. coli-derived recombinant human LNK/SH2B3
Gln427-Leu575
Accession # Q9UQQ2

Formulation

Supplied 0.2mg/ml in 1X PBS with RDF1 and 0.09% Sodium Azide

Label

Alexa Fluor 700 (Excitation= 675-700 nm, Emission= 723 nm)

Applications

Recommended Concentration

Sample

Western Blot

Optimal dilution of this antibody should be experimentally determined.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied

Background: LNK/SH2B3

LNK (also SH2B adaptor protein 3) is a protein intermediary, or LiNK, between cell surface molecules (TPO R; EPO R; SCFR; TCR) and downstream signaling molecules such as PI3 K, Grb2 and Jak2. Although its predicted MW is 63 kDa, it runs anomalously at 75 kDa in SDS‑PAGE. LNK is found in hematopoietic cells, and serves to limit responsiveness to growth factor stimulation. Human LNK is 575 amino acids (aa) in length. It contains an N-terminal Phe-zipper segment that likely serves as a dimerization domain (aa 24‑81), a PH domain that may interact with membrane lipids (aa 197‑305), and an SH2 domain that binds phosphoTyr (aa 364‑462).

Long Name

Lymphocyte Adapter Protein

Entrez Gene IDs

10019 (Human); 16923 (Mouse)

Alternate Names

IDDM20; LNK; LNKSH2B adapter protein 3; Lymphocyte adapter protein; Lymphocyte-specific adapter protein Lnk; SH2B adaptor protein 3; SH2B3; Signal transduction protein Lnk