Human LRP-5 Antibody

R&D Systems | Catalog # AF6646

R&D Systems

Key Product Details

Species Reactivity

Human

Applications

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Sheep IgG
View all LRP-5 Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant human LRP-5
Phe1422-Ser1615
Accession # O75197

Specificity

Detects human LRP-5 in Western blots.

Clonality

Polyclonal

Host

Sheep

Isotype

IgG

Scientific Data Images for Human LRP-5 Antibody

Detection of Human LRP-5 antibody by Western Blot.

Detection of Human LRP-5 by Western Blot.

Western blot shows lysates of HepG2 human hepatocellular carcinoma cell line. PVDF Membrane was probed with 1 µg/mL of Human LRP-5 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6646) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for LRP-5 at approximately 185 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Applications for Human LRP-5 Antibody

Application
Recommended Usage

Western Blot

1 µg/mL
Sample: HepG2 human hepatocellular carcinoma cell line

Reviewed Applications

Read 1 review rated 4 using AF6646 in the following applications:

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Sterile PBS to a final concentration of 0.2 mg/mL. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: LRP-5

LRP-5 (low-density lipoprotein receptor-related protein 5) is a 1615 amino acid (aa), ~180‑200 kDa type I transmembrane glycoprotein. The 1353 aa extracellular domain (ECD) contains 20 class B LDLR, 4 EGF‑like, and 3 class A LDLR repeats. Human LRP-5 aa 745-1097 encompasses class B LDLR repeats #12-17 and EGF‑like repeat #3. It shares 94% aa identity with mouse and rat LRP-5, and 70% aa identity with human LRP-6 within corresponding regions. LRP-5 and LRP-6 mediate DKK effects on Wnt signaling. LRP-5 polymorphisms correlate with susceptibility and resistance to osteoporosis.

Long Name

LDL Receptor-related Protein 5

Alternate Names

BMND1, EVR1, EVR4, HBM, LRP5, LRP7, OPPG, OPS, OPTA1, VBCH2

Entrez Gene IDs

4041 (Human); 16973 (Mouse); 293649 (Rat)

Gene Symbol

LRP5

UniProt

O75197 (Human)

Additional LRP-5 Products

Product Documents for Human LRP-5 Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human LRP-5 Antibody

For research use only

Customer Reviews for Human LRP-5 Antibody (1)

4 out of 5
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  • Human LRP-5 Antibody
    Name: Anonymous
    Application: Western Blot
    Sample Tested: Recombinant protein
    Species: Human
    Verified Customer | Posted 07/07/2021
    Human LRP-5 Antibody AF6646

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