Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human, Mouse

Applications

Validated:

Neutralization

Cited:

Western Blot, Neutralization

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # 135125
View all Lymphotoxin Primary Antibodies »

Product Specifications

Immunogen

S. frugiperda insect ovarian cell line Sf 21-derived recombinant human Lymphotoxin  alpha / beta

Specificity

Recognizes human Lymphotoxin alpha chains in alpha 2 beta 1 or alpha 1 beta 2 trimers and also human TNF-beta. In direct ELISAs, less than 10% cross-reactivity with recombinant human (rh) APRIL, rhBAFF, rhEDA-A2, rhFas Ligand, rhLIGHT, rhGITR Ligand, rhOX40 Ligand, recombinant mouse RANKL, rhTNF-alpha, rhTRAIL, rhTRANCE, rhTWEAK, or rhVEGI is observed.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Endotoxin Level

<0.10 EU per 1 μg of the antibody by the LAL method.

Scientific Data Images for Human Lymphotoxin Antibody

Neutralization of Lymphotoxin alpha 2/ beta 1 Bioactivity by Human Lymphotoxin Antibody

Mouse Anti-Human Lymphotoxin Antibody Clone # 135125 (Catalog # MAB1370) neutralizes Recombinant Human Lymphotoxin alpha 2/ beta 1 (679-TX) induced cytotoxicity in the L-929 mouse fibroblast cell line in the presence of actinomycin D. The ND50 for this effect is typically 0.150-3.00 µg/mL.

Applications for Human Lymphotoxin Antibody

Application
Recommended Usage

Neutralization

Measured by its ability to neutralize Lymphotoxin alpha 2/ beta 1­induced cytotoxicity in the L­929 mouse fibroblast cell line. The Neutralization Dose (ND50) is typically 0.150 - 3.00 µg/mL in the presence of 10 ng/mL Recombinant Human Lymphotoxin alpha 2/ beta 1and actinomycin D.

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. See Certificate of Analysis for details.
*Small pack size (-SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Lymphotoxin

Lymphotoxin alpha, also known as tumor necrosis factor (TNF) beta, and Lymphotoxin beta are members of the TNF superfamily and have been designated TNFSF1 and TNFSF3, respectively. Secreted Lymphotoxin alpha forms a homotrimer (TNF­beta) and heterotrimeric complexes with the type II membrane protein, Lymphotoxin beta (either alpha 2 beta 1or alpha 1 beta 2 timers). These trimers serve as ligands for Lymphotoxin beta receptor, TNF RI and TNF RII and play a role in the development and maturation of the immune system.

References

  1. Matthews, N. and M. L. Neale (1987) in Lymphokines and Interferons, A Practical Approach.
  2. Clemens, M. J. et al. (eds): IRL Press. 221.

Alternate Names

LT, Lymphotoxin alpha, tnfb, TNFSF1, TNLG1E

Entrez Gene IDs

4049 (Human); 16992 (Mouse); 102138497 (Cynomolgus Monkey)

Gene Symbol

LTA

Additional Lymphotoxin Products

Product Documents for Human Lymphotoxin Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

Download SDS

Product Specific Notices for Human Lymphotoxin Antibody

For research use only

Citations for Human Lymphotoxin Antibody

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